LOCUS       sequence-138525-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains a Cas9 Activator for yeast. The activator is 
            about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It
            is on a single copy CEN/ARS plasmid with Ura marker, pRS416..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Naranjo S, Smith JD, Artieri CG, Zhang M, Zhou Y, Palmer ME, Fraser 
            HB
  TITLE     Dissecting the Genetic Basis of a Complex cis-Regulatory Adaptation.
  JOURNAL   PLoS Genet. 2015 Dec 29;11(12):e1005751. doi: 
            10.1371/journal.pgen.1005751. eCollection 2015 Dec.
  PUBMED    26713447
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-12-29"; volume: "11(12):e1005751. doi"; pages: 
            " 10.1371/journal.pgen.1005751. eCollection 2015 Dec"
COMMENT     Sequence Label: pRS416-Gal4-dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..150)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 aagcatgtct aagtcgaaat cgtccagagc gtctgatcca agcatatcca ggtcaaaatc
       61 atctaaggcg tcacttccaa gcatgtcgag gtcaaagtca tctagcgcat cagaacccag
      121 catatccaag tcgaagtcgt ccaaagcgtc agaacctcca ccgtcgactc cagatctacc
      181 agaaccgtca gccctgctgt ctccaccgag ctgagagagg tcgattcttg tttcatagag
      241 ccccgtaatt gactgatgaa tcagtgtggc gtccaggacc tcctttgtag aggtgtaccg
      301 ctttctgtct atggtggtgt cgaagtactt gaaggctgca ggcgcgccca agttggtcag
      361 agtaaacaag tggataatgt tttctgcctg ctccctgatg ggcttatccc tgtgcttatt
      421 gtaagcagaa agcaccttat cgaggttagc gtcggcgagg atcactcttt tggagaattc
      481 gcttatttgc tcgatgatct catcaaggta gtgtttgtgt tgttccacga acagctgctt
      541 ctgctcatta tcttcgggag accctttgag cttttcatag tggctggcca gatacaagaa
      601 attaacgtat ttagagggca gtgccagctc gttacctttc tgcagctcgc ccgcactagc
      661 gagcattcgt ttccggccgt tttcaagctc aaagagagag tacttgggaa gcttaatgat
      721 gaggtctttt ttgacctctt tatatccttt cgcctcgaga aagtcgatgg ggttt
//
