LOCUS       sequence-138528-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 
            promoter, and tet-incucibile RPR1 promoter with NotI cloning site 
            adjacent to gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Smith JD, Suresh S, Schlecht U, Wu M, Wagih O, Peltz G, Davis RW, 
            Steinmetz LM, Parts L, St Onge RP
  TITLE     Quantitative CRISPR interference screens in yeast identify 
            chemical-genetic interactions and new rules for guide RNA design.
  JOURNAL   Genome Biol. 2016 Mar 8;17(1):45. doi: 10.1186/s13059-016-0900-9.
  PUBMED    26956608
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2016-03-8"; volume: "17(1):45. doi"; pages: " 
            10.1186/s13059-016-0900-9"
COMMENT     Sequence Label: pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..84
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttctaga gatgtctaga
       61 gccccaaaga agaagagaaa agttagaccc gggatggaca agaagtactc cattgggctc
      121 gctatcggca caaacagcgt cggctgggcc gtcattacgg acgagtacaa ggtgccgagc
      181 aaaaaattca aagttctggg caataccgat cgccacagca taaagaagaa cctcattggc
      241 gccctcctgt tcgactccgg ggagacggcc gaagccacgc ggctcaaaag aacagcacgg
      301 cgcagatata cccgcagaaa gaatcggatc tgctacctgc aggagatctt tagtaatgag
      361 atggctaagg tggatgactc tttcttccat aggctggagg agtccttttt ggtggaggag
      421 gataaaaagc acgagcgcca cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat
      481 gaaaagtacc caaccatata tcatctgagg aagaagcttg tagacagtac tgataaggct
      541 gacttgcggt tgatctatct cgcgctggcg catatgatca aatttcgggg acacttcctc
      601 atcgaggggg acctgaaccc agacaacagc gatgtcgaca aactctttat ccaactggtt
      661 cagacttaca atcagctttt cgaagagaac ccgatcaacg catccggagt tgacgccaaa
      721 gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
//
