LOCUS       sequence-138531-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR
  TITLE     Pathway engineered enzymatic de novo purine nucleotide synthesis.
  JOURNAL   ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.
  PUBMED    18707057
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2008-08-15- 15"; volume: "3"; issue: "8"; 
            pages: "499-511"
COMMENT     Sequence Label: pET22-HT/plsA
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     266..285
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     369..388
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        369..387
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    388..412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             427..449
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             460..477
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             481..501
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
       61 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
      121 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
      181 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
      241 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
      301 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
      361 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      421 aataattttg tttaacttta agaaggagat atacatatgc atcatcatca tcatcacggc
      481 gaaaacctgt attttcaggg cggatccatg cgtatcattc tgcttggcgc tccgggcgcg
      541 gggaaaggga ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact
      601 ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac
      661 attatggatg ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc
      721 attgctcagg aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg
      781 caggcagacg cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta
      841 ccggacgaac tgattgttga tcgtatcgt
//
