LOCUS       sequence-138535-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in E. coli, E. coli Dihydroneopterin triphosphate 
            pyrophosphatase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Gabelli SB, Bianchet MA, Xu W, Dunn CA, Niu ZD, Amzel LM, Bessman MJ
  TITLE     Structure and function of the E. coli dihydroneopterin triphosphate 
            pyrophosphatase: a Nudix enzyme involved in folate biosynthesis.
  JOURNAL   Structure. 2007 Aug;15(8):1014-22.
  PUBMED    17698004
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Structure."; date: "2007-08"; volume: "15(8)"; pages: "1014-22"
COMMENT     Sequence Label: pET11_DHNTPase
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(541..559)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      555..602
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(777..782,783..799,800..805))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ctttaagaag gagatataca tatgaaggat aaagtgtata agcgtcccgt ttcgatctta
       61 gtggtcatct acgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc
      121 gatttctggc agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc
      181 atgcgcgaag taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt
      241 gactgtcagc gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg
      301 ggcgtgacgc gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcggcagatc
      361 gttttcactg aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc ggcgctcact
      421 aagtcctgga gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc
      481 agggctatct ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      541 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      601 tgctgaaagg aggaactata tccggatatc ccgcaagagg cccggcagta ccggcataac
      661 caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt
      721 agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta
      781 aagcttatcg atgataagct gtcaaa
//
