LOCUS       sequence-138536-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 vector with multi cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 240 pTol2005b
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..71
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     55..71
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(105..116,117..304)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgctctaga
       61 actagtggat ctgctgggct tgctgaaggt agggggtcaa gaaccagagg tgtaaagtac
      121 ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt ttactttact
      181 tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt tagaaaaaaa
      241 agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt tttggagatc
      301 acttcattct attttccctt gctattacca aaccaattga attgcgctga tgcccagttt
      361 aatttaaatg ttatttattc tgcctatgaa aatcgttttc acattatatg aaattggtca
      421 gacatgttca ttggtccttt ggaagtgacg tcatgtcaca tctattacca caatgcacag
      481 caccttgacc tggaaattag ggaaattata acagtcaatc agtggaagaa aatggaggaa
      541 gtatgtgatt catcagcagc tgcgagcagc acagtccaaa atcagccaca ggatcaagag
      601 cacccgtggc cgtatcttcg cgaattcttt tctttaagtg gtgtaaataa agattcattc
      661 aagatgaaat gtgtcctctg tctcccgctt aataaagaaa tatcggcctt caaaagttcg
      721 ccatcaaacc taaggaagca tattgaggta agtacattaa gtatt
//
