LOCUS       sequence-138537-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in E. coli, E. coli Dihydroneopterin triphosphate 
            pyrophosphatase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Gabelli SB, Bianchet MA, Xu W, Dunn CA, Niu ZD, Amzel LM, Bessman MJ
  TITLE     Structure and function of the E. coli dihydroneopterin triphosphate 
            pyrophosphatase: a Nudix enzyme involved in folate biosynthesis.
  JOURNAL   Structure. 2007 Aug;15(8):1014-22.
  PUBMED    17698004
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Structure."; date: "2007-08"; volume: "15(8)"; pages: "1014-22"
COMMENT     Sequence Label: pET11_DHNTPase
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     361..380
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatcctc atttaatggt aaaggtatca tccaccagaa ccacgccgcc
      121 cggcacctgc accggtttcg gtttcatttt acggctgaac aagagtccac tattaaagaa
      181 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
      241 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
      301 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
      361 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
      421 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccaat
      481 ccggatatag ttcctccttt cagcaaaaaa cccctcaaga cccgtttaga ggccccaagg
      541 ggttatgcct agttatt
//
