LOCUS       sequence-138539-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in E. coli, E coli MutT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Bessman MJ, Frick DN, O'Handley SF
  TITLE     The MutT proteins or ""Nudix"" hydrolases, a family of versatile, 
            widely distributed, ""housecleaning"" enzymes.
  JOURNAL   J Biol Chem. 1996 Oct 11;271(41):25059-62.
  PUBMED    8810257
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1996-10-11"; volume: "271(41)"; pages: "25059-62"
COMMENT     Sequence Label: pET11b_MutT
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(486..504)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      500..547
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(722..727,728..744,745..750))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatataca tatgaaaaag ctgcaaattg
       61 cggtaggtat tattcgcaac gagaacaatg aaatctttat aacgcgtcgc gcagcagatg
      121 cgcacatggc gaataaactg gagtttcccg gcggtaaaat tgaaatgggt gaaacgccgg
      181 aacaggcggt ggtgcgtgaa cttcaggaag aagtcgggat taccccccaa catttttcgc
      241 tatttgaaaa actggaatat gaattcccgg acaggcatat aacactgtgg ttttggctgg
      301 tcgaacgctg ggaaggggag ccgtggggta aagaagggca acccggtgag tggatgtcgc
      361 tggtcggtct taatgccgat gattttccgc cagccaatga accggtaatt gcgaagctta
      421 aacgtctgta agtcaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      481 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      541 ttttttgctg aaaggaggaa ctatatccgg atatcccgca agaggcccgg cagtaccggc
      601 ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca
      661 ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg
      721 cattaaagct tatcgatgat aagctgtcaa acatgagaat tctt
//
