LOCUS       sequence-138540-         342 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in E. coli, E coli MutT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Bessman MJ, Frick DN, O'Handley SF
  TITLE     The MutT proteins or ""Nudix"" hydrolases, a family of versatile, 
            widely distributed, ""housecleaning"" enzymes.
  JOURNAL   J Biol Chem. 1996 Oct 11;271(41):25059-62.
  PUBMED    8810257
REFERENCE   2  (bases 1 to 342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1996-10-11"; volume: "271(41)"; pages: "25059-62"
COMMENT     Sequence Label: pET11b_MutT
FEATURES             Location/Qualifiers
     source          1..342
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             228..250
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    265..289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(289..308)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(290..308)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 catattccag tttttcaaat agcgaaaaat gttggggggt aatcccgact tcttcctgaa
       61 gttcacgcac caccgcctgt tccggcgttt cacccatttc aattttaccg ccgggaaact
      121 ccagtttatt cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt tcattgttct
      181 cgttgcgaat aatacctacc gcaatttgca gctttttcat atgtatatct ccttcttaaa
      241 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
      301 tcgtattaat ttcgcgggat cgagatctcg atcctctaca cc
//
