LOCUS       sequence-138562-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized 
            roGFP; human Grx1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Morgan B, Ezerina D, Amoako TN, Riemer J, Seedorf M, Dick TP
  TITLE     Multiple glutathione disulfide removal pathways mediate cytosolic 
            redox homeostasis.
  JOURNAL   Nat Chem Biol. 2013 Feb;9(2):119-25. doi: 10.1038/nchembio.1142. 
            Epub 2012 Dec 16.
  PUBMED    23242256
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1142"; journalName: "Nat Chem Biol"; date: 
            "2013-02"; volume: "9"; issue: "2"; pages: "119-25"
COMMENT     Sequence Label: p415TEF cyto roGFP(iL)-Grx1(human)
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..411
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 aaagctggag ctcatagctt caaaatgttt ctactccttt tttactcttc cagattttct
       61 cggactccgc gcatcgccgt accacttcaa aacacccaag cacagcatac taaatttccc
      121 ctctttcttc ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag
      181 agaccgcctc gtttcttttt cttcgtcgaa aaaggcaata aaaattttta tcacgtttct
      241 ttttcttgaa aatttttttt ttgatttttt tctctttcga tgacctccca ttgatattta
      301 agttaataaa cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa
      361 ctttttttac ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttctaga
      421 ggatccacca tggctagcga attctctaaa ggtgaagaat tgttcacagg tgtagtccct
      481 attttggtcg aattggatgg tgacgtaaac ggtcataagt tctcagtatc aggtgaaggt
      541 gaaggtgacg caacatacgg taaattgacc ttgaagttta tttcaactac tggtaaattg
      601 ccagttcctt ggccaacttt ggtaaccact ttaagttatg gtgtacaatg tttctcaaga
      661 taccctgatc atatgaaaca acacgacttt ttcaagtctg ctatgccaga aggttacgtt
      721 caagaaagaa caattttctt taaggatgac ggtaactaca agactagagc agaagtcaaa
      781 tttgaaggtg acacattggt taacagaatc gaattgaagg gtatcgattt caaggaagac
      841 ggtaacatct tgggtcataa attggaatac aactacaact gtttgtcaaa cgtatacatc
      901 atggccgata agcaaa
//
