LOCUS       sequence-138567-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized 
            roGFP; human Grx1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Morgan B, Ezerina D, Amoako TN, Riemer J, Seedorf M, Dick TP
  TITLE     Multiple glutathione disulfide removal pathways mediate cytosolic 
            redox homeostasis.
  JOURNAL   Nat Chem Biol. 2013 Feb;9(2):119-25. doi: 10.1038/nchembio.1142. 
            Epub 2012 Dec 16.
  PUBMED    23242256
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1142"; journalName: "Nat Chem Biol"; date: 
            "2013-02"; volume: "9"; issue: "2"; pages: "119-25"
COMMENT     Sequence Label: p415TEF cyto roGFP(iL)-Grx1(human)
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     516..534
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(572..594)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     694..713
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa
       61 aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc
      121 ttttatcttg ctcttcctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt
      181 agaagaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat
      241 atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga
      301 aattttttaa acctttgttt attttttttt cttcattccg taactcttct accttcttta
      361 tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca
      421 aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatccgtcct
      481 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
      541 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      601 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      661 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
      721 tgcaccatat cgactacgtc gttaaggccg tttctgacag agtaaaattc ttgagggaac
      781 tttcaccatt atgggaa
//
