LOCUS       sequence-138575-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  J8AAV-HDR U6sgCtnnb1.1 template.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Yin H, Song CQ, Dorkin JR, Zhu LJ, Li Y, Wu Q, Park A, Yang J, 
            Suresh S, Bizhanova A, Gupta A, Bolukbasi MF, Walsh S, Bogorad RL, 
            Gao G, Weng Z, Dong Y, Koteliansky V, Wolfe SA, Langer R, Xue W, 
            Anderson DG
  TITLE     Therapeutic genome editing by combined viral and non-viral delivery 
            of CRISPR system components in vivo.
  JOURNAL   Nat Biotechnol. 2016 Feb 1. doi: 10.1038/nbt.3471.
  PUBMED    26829318
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Feb 1. doi: 10.1038/nbt.3471."
COMMENT     Sequence Label: J8AAV-HDR Ctnnb1.1
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        224..299
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccgctgtggt ggtggcacca gaagttttag agctagaaat
      241 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
      301 tttttgtttt agagctagaa atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg
      361 cgccaattct gcagacaaat ggctttccct agagagggga cattctctag aagcaccgta
      421 tgcctacaat ctgtttctac tgagaagagc ttcctgacac cgtggctgct gtgtattttt
      481 agtgtatgcc atggtgaact ggcttttggt gtcggggcac atagcctagt ataggtagca
      541 gaatcacggt gacctgggtt aaaaacaaac ttaagtttta atgacttgat ggaatttttc
      601 agggtacctg aagctcagcg cacagctgct gtgacaccgc tgcgtggaca atggctactc
      661 aaggtttgtg attcagatgc ctgtctgagg atctgcctca tagccctgct gcctggccag
      721 actgcctttg ttctcttccc ttctgcacac taccactggc ttggtgaaa
//
