LOCUS       sequence-138577-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  J8AAV-HDR U6sgCtnnb1.1 template.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Yin H, Song CQ, Dorkin JR, Zhu LJ, Li Y, Wu Q, Park A, Yang J, 
            Suresh S, Bizhanova A, Gupta A, Bolukbasi MF, Walsh S, Bogorad RL, 
            Gao G, Weng Z, Dong Y, Koteliansky V, Wolfe SA, Langer R, Xue W, 
            Anderson DG
  TITLE     Therapeutic genome editing by combined viral and non-viral delivery 
            of CRISPR system components in vivo.
  JOURNAL   Nat Biotechnol. 2016 Feb 1. doi: 10.1038/nbt.3471.
  PUBMED    26829318
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Feb 1. doi: 10.1038/nbt.3471."
COMMENT     Sequence Label: J8AAV-HDR Ctnnb1.1
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        35..110
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aggacgaaac accgctgtgg tggtggcacc agaagtttta gagctagaaa tagcaagtta
       61 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt
      121 tagagctaga aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcgccaattc
      181 tgcagacaaa tggctttccc tagagagggg acattctcta gaagcaccgt atgcctacaa
      241 tctgtttcta ctgagaagag cttcctgaca ccgtggctgc tgtgtatttt tagtgtatgc
      301 catggtgaac tggcttttgg tgtcggggca catagcctag tataggtagc agaatcacgg
      361 tgacctgggt taaaaacaaa cttaagtttt aatgacttga tggaattttt cagggtacct
      421 gaagctcagc gcacagctgc tgtgacaccg ctgcgtggac aatggctact caaggtttgt
      481 gattcagatg cctgtctgag gatctgcctc atagccctgc tgcctggcca gactgccttt
      541 gttctcttcc cttctgcaca ctaccactgg cttggtgaaa taatcagcaa gccaccgatg
      601 ggatctaatg agtgctctca ggccacccct gtcccatgga gctcatactg accctcacct
      661 gctctccttg gctgccttct aacagtattt caatgggtca ttgtcgtaga tggcttcttc
      721 aggtagcatt ttcagttcac taacatactc tgtttttaca gctgacctga tggagttgg
//
