LOCUS       sequence-138585-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  standard plasmids for exogenus pathway assembly haboring yeast 
            auxotrophic marker TRP1 which could be released and ligated to 
            series of TUs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pMV-TRP1
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(87..761)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 gcgtctcttg agctgtgcgg tatttcacac cgcatagatc ggcaagtgca caaacaatac
       61 ttaaataaat actactcagt aataacctat ttcttagcat ttttgacgaa atttgctatt
      121 ttgttagagt cttttacacc atttgtctcc acacctccgc ttacatcaac accaataacg
      181 ccatttaatc taagcgcatc accaacattt tctggcgtca gtccaccagc taacataaaa
      241 tgtaagcttt cggggctctc ttgccttcca acccagtcag aaatcgagtt ccaatccaaa
      301 agttcacctg tcccacctgc ttctgaatca aacaagggaa taaacgaatg aggtttctgt
      361 gaagctgcac tgagtagtat gttgcagtct tttggaaata cgagtctttt aataactggc
      421 aaaccgagga actcttggta ttcttgccac gactcatctc catgcagttg gacgatatca
      481 atgccgtaat cattgaccag agccaaaaca tcctccttag gttgattacg aaacacgcca
      541 accaagtatt tcggagtgcc tgaactattt ttatatgctt ttacaagact tgaaattttc
      601 cttgcaataa ccgggtcaat tgttctcttt ctattgggca cacatataat acccagcaag
      661 tcagcatcgg aatctagagc acattctgcg gcctctgtgc tctgcaagcc gcaaactttc
      721 accaatggac cagaactacc tgtgaaatta ataacagaca tactccaagc tgcctttgtg
      781 tgcttaatca cgtatactca cgtgctcaat agtcaccaat gcc
//
