LOCUS       sequence-138593-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK368
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             555..572
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(625..643)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      639..686
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(810..829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgtcagaa acctatggca ccggccagaa
       61 ggaacagcac tgcatgcccc gctcagaaaa ttcccaagcc ccaaccgata tccccaccat
      121 ccgcgtggtg gccatgccca cagacaccaa cccggcggga gacgtgtttg gcgggtggat
      181 tgtttctcag atggatcttg cagccggcac cacggcggcg tttcgggcca atggccggtg
      241 cgccaccatt gccattaaca gccttgtgtt tatggaaccc gtgatggtgg gggatgaagt
      301 gagcatttac acccgcattt tgcgcgcggg gcgtacatcg ctcaccattc atgttgaaac
      361 atggcgccgg gcccgccaca cgcatctgac atccaaggtg acggaaggcg agtttatttt
      421 tgtggcgctg gatgaaaacc gccgccctcg ccagctgcca cccccgcagc cgggtgatga
      481 actgccagaa attgtacccc ccaacgcacg ctaggaattc gagctccgtc gacaagcttg
      541 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      601 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      661 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      721 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      901 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      961 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt t
//
