LOCUS       sequence-138594-        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK368
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             578..600
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(631..650)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(632..650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattccta gcgtgcgttg gggggtacaa tttctggcag
      121 ttcatcaccc ggctgcgggg gtggcagctg gcgagggcgg cggttttcat ccagcgccac
      181 aaaaataaac tcgccttccg tcaccttgga tgtcagatgc gtgtggcggg cccggcgcca
      241 tgtttcaaca tgaatggtga gcgatgtacg ccccgcgcgc aaaatgcggg tgtaaatgct
      301 cacttcatcc cccaccatca cgggttccat aaacacaagg ctgttaatgg caatggtggc
      361 gcaccggcca ttggcccgaa acgccgccgt ggtgccggct gcaagatcca tctgagaaac
      421 aatccacccg ccaaacacgt ctcccgccgg gttggtgtct gtgggcatgg ccaccacgcg
      481 gatggtgggg atatcggttg gggcttggga attttctgag cggggcatgc agtgctgttc
      541 cttctggccg gtgccatagg tttctgacat atgtatatct ccttcttaaa gttaaacaaa
      601 attatttcta gagggaaacc gttgtggtct ccctatagtg agtcgtatta atttcgcggg
      661 atcgagatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg
      721 ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata
      781 cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga
      841 atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc
      901 attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct
      961 gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat
     1021 accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc
     1081 cgtatcgtga gcatcctctc tcgtttcatc gg
//
