LOCUS       sequence-13860-e        6313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6313)
  TITLE     Ramakrishnan Lab Plasmids
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pR2HYG
FEATURES             Location/Qualifiers
     source          1..6313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(129..148)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(213..232)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2323..2911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2812..2831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4974..4990
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4974..4990
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5555..5571)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5556..5572)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             5626..6303
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(5806..5826)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6218..6241
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tctagccacc tgacgtcggg ggggggggga aagccacgtt gtgtctcaaa atctctgatg
       61 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      121 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg
      181 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      241 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc catgcgccag agttgtttct
      301 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      361 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgacta
      421 gagcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      481 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      541 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccga
      601 cgtggccgac cagcccgtca tcgtcaacgc ctgatccgcg gtgcggacag gccgtgtcgc
      661 gaccggccgt gcggaattaa gccggcccgt accctgtgaa tagaggtccg ctgtgacaca
      721 agaatccctg ttacttctcg accgtattga ttcggatgat tcctacgcga gcctgcggaa
      781 cgaccaggaa ttctgggagc cgctggcccg ccgagccctg gaggagctcg ggctgccggt
      841 gccgccggtg ctgcgggtgc ccggcgagag caccaacccc gtactggtcg gcgagcccgg
      901 cccggtgatc aagctgttcg gcgagcactg gtgcggtccg gagagcctcg cgtcggagtc
      961 ggaggcgtac gcggtcctgg cggacgcccc ggtgccggtg ccccgcctcc tcggccgcgg
     1021 cgagctgcgg cccggcaccg gagcctggcc gtggccctac ctggtgatga gccggatgac
     1081 cggcaccacc tggcggtccg cgatggacgg cacgaccgac cggaacgcgc tgctcgccct
     1141 ggcccgcgaa ctcggccggg tgctcggccg gctgcacagg gtgccgctga ccgggaacac
     1201 cgtgctcacc ccccattccg aggtcttccc ggaactgctg cgggaacgcc gcgcggcgac
     1261 cgtcgaggac caccgcgggt ggggctacct ctcgccccgg ctgctggacc gcctggagga
     1321 ctggctgccg gacgtggaca cgctgctggc cggccgcgaa ccccggttcg tccacggcga
     1381 cctgcacggg accaacatct tcgtggacct ggccgcgacc gaggtcaccg ggatcgtcga
     1441 cttcaccgac gtctatgcgg gagactcccg ctacagcctg gtgcaactgc atctcaacgc
     1501 cttccggggc gaccgcgaga tcctggccgc gctgctcgac ggggcgcagt ggaagcggac
     1561 cgaggacttc gcccgcgaac tgctcgcctt caccttcctg cacgacttcg aggtgttcga
     1621 ggagaccccg ctggatctct ccggcttcac cgatccggag gaactggcgc agttcctctg
     1681 ggggccgccg gacaccgccc ccggcgcctg acgccccctc tagtaatctt ttgccattct
     1741 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     1801 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     1861 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     1921 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     1981 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     2041 gacgggacgg cggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac
     2101 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg
     2161 gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg
     2221 ggcgattcag gcctggtatg agtcagcaac accttcttca cgaggcagac ctcactagtt
     2281 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2341 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2401 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2461 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2521 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2581 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2641 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2701 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2761 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2821 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2881 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2941 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3001 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3061 gcgcaacgcg tgagcccacc agctccgtaa gttcgggtgc tgtgtggctc gtacccgcgc
     3121 attcaggcgg cagggggtct aacgggtcta aggcggcgtg tacggccgcc acagcggctc
     3181 ttagcggccc ggaaacgtcc tcgaaacgac gcatgtgttc ctcctggttg gtacaggtgg
     3241 ttgggggtgc tcggctgtcg ctggtgtttc atcatcaggg ctcgacggga gagcggggga
     3301 gtgtgcagtt gtggggtggc ccctcagcga aatatctgac ttggagctcg tgtcggacca
     3361 tacaccggtg attaatcgtg gtttattatc aagcgtgagc cacgtcgccg acgaatttga
     3421 gcagctctgg ctgccgtact ggtccctggc aagcgacgat ctgctcgagg ggatctaccg
     3481 ccaaagccgc gcgtcggccc taggccgccg gtacatcgag gcgaacccaa cagcgctggc
     3541 aaacctgctg gtcgtggacg tagaccatcc agacgcagcg ctccgagcgc tcagcgcccg
     3601 ggggtcccat ccgctgccca acgcgatcgt gggcaatcgc gccaacggcc acgcacacgc
     3661 agtgtgggca ctcaacgccc ctgttccacg caccgaatac gcgcggcgta agccgctcgc
     3721 atacatggcg gcgtgcgccg aaggccttcg gcgcgccgtc gatggcgacc gcagttactc
     3781 aggcctcatg accaaaaacc ccggccacat cgcctgggaa acggaatggc tccactcaga
     3841 tctctacaca ctcagccaca tcgaggccga gctcggcgcg aacatgccac cgccgcgctg
     3901 gcgtcagcag accacgtaca aagcggctcc gacgccgcta gggcggaatt gcgcactgtt
     3961 cgattccgtc aggttgtggg cctatcttcc cgccctcatg cggatctacc tgccgacccg
     4021 gaacgtggac ggactcggcc gcgcgatcta tgccgagtgc cacgcgcgaa acgccgaatt
     4081 tccgtgcaac gacgtgtgtc ccggaccgct accggacagc gaggtccgcg ccatcgccaa
     4141 cagcatttgg cgttggatca caaccaagtc gcgcatttgg gcggacggga tcgtggtcta
     4201 cgaggccaca ctcagtgcgc gccatgcggc catctcgcgg aagggcgcag cagcgcgcac
     4261 ggcggcgagc acagttgcgc ggcgcgcaaa gtccgcgtca gccatggagg cattgctatg
     4321 agcgacggct acagcgacgg ctacagcgac ggctacaact ggcagccgac tgtccgcaaa
     4381 aagcggcgcg tgaccgccgc cgaaggcgct cgaatcaccg gactatccga acgccacgtc
     4441 gtccggctcg tggcgcagga acgcagcgag tggttcgccg agcaggctgc acgccgcgaa
     4501 cgcatccgcg cctatcacga cgacgagggc cactcttggc cgcaaacggc caaacatttc
     4561 gggctgcatc tggacaccgt taagcgactc ggctatcggg cgaggaaaga gcgtgcggca
     4621 gaacaggaag cggctcaaaa ggcccacaac gaagccgaca atccaccgct gttctaacgc
     4681 aattggggag cgggtgtcgc gggggttccg tggggggttc cgttgcaacg ggtcggacag
     4741 gtaaaagtcc tggtagacgc tagttttctg gtttgggcca tgcctgtctc gttgcgtgtt
     4801 tcgttgcgtc cgttttgaat accagccaga cgagacgggg ttctacgaat cttggtcgat
     4861 accaagccat ttccgctgaa tatcgtggag ctcaccgcca gaatcggtgg ttgtggtgat
     4921 gtacgtggcg aactccgttg tagtgcttgt ggtggcatcc gtggcgcggc cgctctagaa
     4981 ctagtggatc tgacccgctc cacaaccgct gagcagggtg gcagtcagag cgaccaggcc
     5041 gaccgatacg aggcggattt tgaacaggtt cactcgaagc ctcccgctgg gttcggagca
     5101 tcgtcgatca catgacggcg tgctgacacc gattcgacta tgcacagtag taggtagccg
     5161 ccgcggacgg tagcgcaggc ggcctcggcg ccgtcgtggc gtcgcgatgg ttggcgccag
     5221 acccgcctga tcggcgtccc gaccagggcc gccggggtgg tgagcctcgg cttggtccag
     5281 ccgtagtgag acgggatgtc ttcccggttg tcgggtttgg gtgggcggtc acatgttcgc
     5341 tggctgtggg cgtgtcgggg gtcgtcgaag cggccgcgaa cagggctgga cgatgcacgt
     5401 cttgcacctc ctgtcaaccc ccaatgttga gccgcgatgc ccctgacctg caccgttgga
     5461 ccgcagtcgt taggggggcg tgttagcatg gagtgacgaa aggggcctaa atcagatgat
     5521 cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc
     5581 ccggtaccag atctttaaat ctagatttaa gaaggagata tacatatggc ctcctccgag
     5641 aacgtcatca ccgagttcat gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac
     5701 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggccacaa caccgtgaag
     5761 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     5821 cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg
     5881 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg
     5941 accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc
     6001 ggcgtgaact tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc
     6061 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagaccca caaggccctg
     6121 aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag
     6181 cccgtgcagc tgcccggcta ctactacgtg gacgccaagc tggacatcac ctcccacaac
     6241 gaggactaca ccatcgtgga gcagtacgag cgcaccgagg gccgccacca cctgttcctg
     6301 tagcggccgc gac
//
