LOCUS       sequence-138604-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est03.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK373
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             486..503
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(556..574)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      570..617
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      654..1082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(741..760)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     951..972
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgaccacc cactccatag atttcagaat
       61 ctattatgaa gatacggatg ctggcggcat tgtgtaccat gcacggtatc tggcttttgc
      121 ggaacgcgcg cgtaccgagg cgattcgtag cctaggtacg ccggcatcac gtttgcagga
      181 agaatatggg ctggtttttg tggtgcatga cgcccatctt gcttacaaaa tgcccctgaa
      241 gctggatgac attgtaacag ttaccacccg cttgattgac ctgaaagccg caagctgccg
      301 actggatcag ctttgcacgc gcggtgatga tgtatgcgca caggttgaag ttggtctggc
      361 ctgtgtgaag gtatcagatg ggcgtcctgc ccgatttccg ccgttatggc gtgatctgtt
      421 gcaaaaattg gcaccaaagg gatgagaatt cgagctccgt cgacaagctt gcggccgcac
      481 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      541 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      601 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
      661 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      721 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      781 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      841 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
      901 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
      961 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     1021 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     1081 ttttaacaaa a
//
