LOCUS       sequence-138605-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est03.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK373
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             501..523
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(554..573)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(555..573)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattct catccctttg gtgccaattt ttgcaacaga tcacgccata
      121 acggcggaaa tcgggcagga cgcccatctg ataccttcac acaggccaga ccaacttcaa
      181 cctgtgcgca tacatcatca ccgcgcgtgc aaagctgatc cagtcggcag cttgcggctt
      241 tcaggtcaat caagcgggtg gtaactgtta caatgtcatc cagcttcagg ggcattttgt
      301 aagcaagatg ggcgtcatgc accacaaaaa ccagcccata ttcttcctgc aaacgtgatg
      361 ccggcgtacc taggctacga atcgcctcgg tacgcgcgcg ttccgcaaaa gccagatacc
      421 gtgcatggta cacaatgccg ccagcatccg tatcttcata atagattctg aaatctatgg
      481 agtgggtggt catatgtata tctccttctt aaagttaaac aaaattattt ctagagggaa
      541 accgttgtgg tctccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgggcag
      601 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
      661 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
      721 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
      781 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg
      841 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg
      901 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc
      961 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
     1021 ctctcgtttc atcggtatca ttacccccat gaacagaaat ccccct
//
