LOCUS       sequence-138608-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLlac* reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pCH8
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             156..185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    320..336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    351..367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cacacaacgt cttggagcgc cagaggagga acgagctaaa acggagcttt tttgccctgc
       61 gtgaccagat cccggagttg gaaaacaatg aaaaggcccc caaggtagtt atccttaaaa
      121 aagccacagc atacatcctg tccgtccaag cagaggagca aaagctcatt tctgaagagg
      181 acttgttgcg gaaacgacga gaacagttga aacacaaact tgaacagcta cggaactctt
      241 gtgcgtaagg aaaagtaagg aaaacgattc cttctaacag aaatgtcctg agcaatcacc
      301 tatgaactgt cgattgacat tgtgagcgga taacaagata ctgagcacaa ttgtgagcgg
      361 ataacaattg catgccactt aaaaaggaga tcaacaatga aagcaatttt cgtactgaaa
      421 catcttaatc atgctaagga gaaatactag tatgcgtaaa ggcgaagagc tgttcactgg
      481 tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg
      541 tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac
      601 tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg
      661 ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga
      721 aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca a
//
