LOCUS       sequence-13861-e        5277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Cosma CL, Humbert O, Ramakrishnan L
  TITLE     Superinfecting mycobacteria home to established tuberculous 
            granulomas.
  JOURNAL   Nat Immunol. 2004 Aug . 5(8):828-35.
  PUBMED    15220915
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Immunol. 2004 Aug . 5(8):828-35."
COMMENT     Sequence Label: pMSP12::dsRed2
FEATURES             Location/Qualifiers
     source          1..5277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(129..148)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             140..955
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(213..232)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1287..1875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1776..1795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3938..3954
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3938..3954
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4519..4535)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4520..4536)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             4590..5267
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(4770..4790)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5182..5205
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tctagccacc tgacgtcggg ggggggggga aagccacgtt gtgtctcaaa atctctgatg
       61 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      121 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg
      181 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      241 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc catgcgccag agttgtttct
      301 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      361 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
      421 atggttactc accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc
      481 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
      541 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
      601 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
      661 tgttgaacaa gtctggaaag aaatgcataa tcttttgcca ttctcaccgg attcagtcgt
      721 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
      781 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
      841 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
      901 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
      961 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcggctt
     1021 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
     1081 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa
     1141 gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcctgg
     1201 tatgagtcag caacaccttc ttcacgaggc agacctcact agttccactg agcgtcagac
     1261 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1321 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1381 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1441 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1501 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1561 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1621 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     1681 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1741 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1801 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1861 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1921 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1981 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa cgcgtgagcc
     2041 caccagctcc gtaagttcgg gtgctgtgtg gctcgtaccc gcgcattcag gcggcagggg
     2101 gtctaacggg tctaaggcgg cgtgtacggc cgccacagcg gctcttagcg gcccggaaac
     2161 gtcctcgaaa cgacgcatgt gttcctcctg gttggtacag gtggttgggg gtgctcggct
     2221 gtcgctggtg tttcatcatc agggctcgac gggagagcgg gggagtgtgc agttgtgggg
     2281 tggcccctca gcgaaatatc tgacttggag ctcgtgtcgg accatacacc ggtgattaat
     2341 cgtggtttat tatcaagcgt gagccacgtc gccgacgaat ttgagcagct ctggctgccg
     2401 tactggtccc tggcaagcga cgatctgctc gaggggatct accgccaaag ccgcgcgtcg
     2461 gccctaggcc gccggtacat cgaggcgaac ccaacagcgc tggcaaacct gctggtcgtg
     2521 gacgtagacc atccagacgc agcgctccga gcgctcagcg cccgggggtc ccatccgctg
     2581 cccaacgcga tcgtgggcaa tcgcgccaac ggccacgcac acgcagtgtg ggcactcaac
     2641 gcccctgttc cacgcaccga atacgcgcgg cgtaagccgc tcgcatacat ggcggcgtgc
     2701 gccgaaggcc ttcggcgcgc cgtcgatggc gaccgcagtt actcaggcct catgaccaaa
     2761 aaccccggcc acatcgcctg ggaaacggaa tggctccact cagatctcta cacactcagc
     2821 cacatcgagg ccgagctcgg cgcgaacatg ccaccgccgc gctggcgtca gcagaccacg
     2881 tacaaagcgg ctccgacgcc gctagggcgg aattgcgcac tgttcgattc cgtcaggttg
     2941 tgggcctatc ttcccgccct catgcggatc tacctgccga cccggaacgt ggacggactc
     3001 ggccgcgcga tctatgccga gtgccacgcg cgaaacgccg aatttccgtg caacgacgtg
     3061 tgtcccggac cgctaccgga cagcgaggtc cgcgccatcg ccaacagcat ttggcgttgg
     3121 atcacaacca agtcgcgcat ttgggcggac gggatcgtgg tctacgaggc cacactcagt
     3181 gcgcgccatg cggccatctc gcggaagggc gcagcagcgc gcacggcggc gagcacagtt
     3241 gcgcggcgcg caaagtccgc gtcagccatg gaggcattgc tatgagcgac ggctacagcg
     3301 acggctacag cgacggctac aactggcagc cgactgtccg caaaaagcgg cgcgtgaccg
     3361 ccgccgaagg cgctcgaatc accggactat ccgaacgcca cgtcgtccgg ctcgtggcgc
     3421 aggaacgcag cgagtggttc gccgagcagg ctgcacgccg cgaacgcatc cgcgcctatc
     3481 acgacgacga gggccactct tggccgcaaa cggccaaaca tttcgggctg catctggaca
     3541 ccgttaagcg actcggctat cgggcgagga aagagcgtgc ggcagaacag gaagcggctc
     3601 aaaaggccca caacgaagcc gacaatccac cgctgttcta acgcaattgg ggagcgggtg
     3661 tcgcgggggt tccgtggggg gttccgttgc aacgggtcgg acaggtaaaa gtcctggtag
     3721 acgctagttt tctggtttgg gccatgcctg tctcgttgcg tgtttcgttg cgtccgtttt
     3781 gaataccagc cagacgagac ggggttctac gaatcttggt cgataccaag ccatttccgc
     3841 tgaatatcgt ggagctcacc gccagaatcg gtggttgtgg tgatgtacgt ggcgaactcc
     3901 gttgtagtgc ttgtggtggc atccgtggcg cggccgctct agaactagtg gatctgaccc
     3961 gctccacaac cgctgagcag ggtggcagtc agagcgacca ggccgaccga tacgaggcgg
     4021 attttgaaca ggttcactcg aagcctcccg ctgggttcgg agcatcgtcg atcacatgac
     4081 ggcgtgctga caccgattcg actatgcaca gtagtaggta gccgccgcgg acggtagcgc
     4141 aggcggcctc ggcgccgtcg tggcgtcgcg atggttggcg ccagacccgc ctgatcggcg
     4201 tcccgaccag ggccgccggg gtggtgagcc tcggcttggt ccagccgtag tgagacggga
     4261 tgtcttcccg gttgtcgggt ttgggtgggc ggtcacatgt tcgctggctg tgggcgtgtc
     4321 gggggtcgtc gaagcggccg cgaacagggc tggacgatgc acgtcttgca cctcctgtca
     4381 acccccaatg ttgagccgcg atgcccctga cctgcaccgt tggaccgcag tcgttagggg
     4441 ggcgtgttag catggagtga cgaaaggggc ctaaatcaga tgatcccccg ggctgcagga
     4501 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta ccagatcttt
     4561 aaatctagat ttaagaagga gatatacata tggcctcctc cgagaacgtc atcaccgagt
     4621 tcatgcgctt caaggtgcgc atggagggca ccgtgaacgg ccacgagttc gagatcgagg
     4681 gcgagggcga gggccgcccc tacgagggcc acaacaccgt gaagctgaag gtgaccaagg
     4741 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg
     4801 tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct
     4861 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg acccaggact
     4921 cctccctgca ggacggctgc ttcatctaca aggtgaagtt catcggcgtg aacttcccct
     4981 ccgacggccc cgtgatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     5041 acccccgcga cggcgtgctg aagggcgaga cccacaaggc cctgaagctg aaggacggcg
     5101 gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg
     5161 gctactacta cgtggacgcc aagctggaca tcacctccca caacgaggac tacaccatcg
     5221 tggagcagta cgagcgcacc gagggccgcc accacctgtt cctgtagcgg ccgcgac
//
