LOCUS       sequence-13862-e        6336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Takaki K, Davis JM, Winglee K, Ramakrishnan L
  TITLE     Evaluation of the pathogenesis and treatment of Mycobacterium 
            marinum infection in zebrafish.
  JOURNAL   Nat Protoc. 2013 Jun;8(6):1114-24. doi: 10.1038/nprot.2013.068. Epub
            2013 May 16.
  PUBMED    23680983
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.068"; journalName: "Nat Protoc"; date: 
            "2013-06"; volume: "8"; issue: "6"; pages: "1114-24"
COMMENT     Sequence Label: pTEC15
FEATURES             Location/Qualifiers
     source          1..6336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             39..749
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
     primer_bind     complement(878..897)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(962..981)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3072..3660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3561..3580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5723..5739
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5723..5739
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6304..6320)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6305..6321)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cagatcttta aatctagatt taagaaggag atatacatat ggtgagcaag ggcgaggaga
       61 ccacaatggg cgtaatcaag cccgacatga agatcaagct gaagatggag ggcaacgtga
      121 atggccacgc cttcgtgatc gagggcgagg gcgagggcaa gccctacgac ggcaccaaca
      181 ccatcaacct ggaggtgaag gagggagccc ccctgccctt ctcctacgac attctgacca
      241 ccgcgttcag ttacggcaac agggccttca ccaagtaccc cgacgacatc cccaactact
      301 tcaagcagtc cttccccgag ggctactctt gggagcgcac catgaccttc gaggacaagg
      361 gcatcgtgaa ggtgaagtcc gacatctcca tggaggagga ctccttcatc tacgagatac
      421 acctcaaggg cgagaacttc ccccccaacg gccccgtgat gcagaaggag accaccggct
      481 gggacgcctc caccgagagg atgtacgtgc gcgacggcgt gctgaagggc gacgtcaaga
      541 tgaagctgct gctggagggc ggcggccacc accgcgttga cttcaagacc atctacaggg
      601 ccaagaaggc ggtgaagctg cccgactatc actttgtgga ccaccgcatc gagatcctga
      661 accacgacaa ggactacaac aaggtgaccg tttacgagat cgccgtggcc cgcaactcca
      721 ccgacggcat ggacgagctg tacaagtaag ctagccacct gacgtcgggg ggggggggaa
      781 agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
      841 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
      901 caacgggaaa cgtcttgctc gaggccgcga ttaaattcca acatggatgc tgatttatat
      961 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     1021 gggaagcccc atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     1081 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     1141 aagcatttta tccgtactcc tgatgactag agcatgagat tatcaaaaag gatcttcacc
     1201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1261 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1321 cgttcatcca tagttgcctg actccccgac gtggccgacc agcccgtcat cgtcaacgcc
     1381 tgatccgcgg tgcggacagg ccgtgtcgcg accggccgtg cggaattaag ccggcccgta
     1441 ccctgtgaat agaggtccgc tgtgacacaa gaatccctgt tacttctcga ccgtattgat
     1501 tcggatgatt cctacgcgag cctgcggaac gaccaggaat tctgggagcc gctggcccgc
     1561 cgagccctgg aggagctcgg gctgccggtg ccgccggtgc tgcgggtgcc cggcgagagc
     1621 accaaccccg tactggtcgg cgagcccggc ccggtgatca agctgttcgg cgagcactgg
     1681 tgcggtccgg agagcctcgc gtcggagtcg gaggcgtacg cggtcctggc ggacgccccg
     1741 gtgccggtgc cccgcctcct cggccgcggc gagctgcggc ccggcaccgg agcctggccg
     1801 tggccctacc tggtgatgag ccggatgacc ggcaccacct ggcggtccgc gatggacggc
     1861 acgaccgacc ggaacgcgct gctcgccctg gcccgcgaac tcggccgggt gctcggccgg
     1921 ctgcacaggg tgccgctgac cgggaacacc gtgctcaccc cccattccga ggtcttcccg
     1981 gaactgctgc gggaacgccg cgcggcgacc gtcgaggacc accgcgggtg gggctacctc
     2041 tcgccccggc tgctggaccg cctggaggac tggctgccgg acgtggacac gctgctggcc
     2101 ggccgcgaac cccggttcgt ccacggcgac ctgcacggga ccaacatctt cgtggacctg
     2161 gccgcgaccg aggtcaccgg gatcgtcgac ttcaccgacg tctatgcggg agactcccgc
     2221 tacagcctgg tgcaactgca tctcaacgcc ttccggggcg accgcgagat cctggccgcg
     2281 ctgctcgacg gggcgcagtg gaagcggacc gaggacttcg cccgcgaact gctcgccttc
     2341 accttcctgc acgacttcga ggtgttcgag gagaccccgc tggatctctc cggcttcacc
     2401 gatccggagg aactggcgca gttcctctgg gggccgccgg acaccgcccc cggcgcctga
     2461 cgccccctct agtaatcttt tgccattctc accggattca gtcgtcactc atggtgattt
     2521 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     2581 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     2641 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     2701 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     2761 gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg aataaatcga
     2821 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     2881 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     2941 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
     3001 ccttcttcac gaggcagacc tcactagttc cactgagcgt cagaccccgt agaaaagatc
     3061 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3181 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3421 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     3481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3721 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3781 gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gagcccacca gctccgtaag
     3841 ttcgggtgct gtgtggctcg tacccgcgca ttcaggcggc agggggtcta acgggtctaa
     3901 ggcggcgtgt acggccgcca cagcggctct tagcggcccg gaaacgtcct cgaaacgacg
     3961 catgtgttcc tcctggttgg tacaggtggt tgggggtgct cggctgtcgc tggtgtttca
     4021 tcatcagggc tcgacgggag agcgggggag tgtgcagttg tggggtggcc cctcagcgaa
     4081 atatctgact tggagctcgt gtcggaccat acaccggtga ttaatcgtgg tttattatca
     4141 agcgtgagcc acgtcgccga cgaatttgag cagctctggc tgccgtactg gtccctggca
     4201 agcgacgatc tgctcgaggg gatctaccgc caaagccgcg cgtcggccct aggccgccgg
     4261 tacatcgagg cgaacccaac agcgctggca aacctgctgg tcgtggacgt agaccatcca
     4321 gacgcagcgc tccgagcgct cagcgcccgg gggtcccatc cgctgcccaa cgcgatcgtg
     4381 ggcaatcgcg ccaacggcca cgcacacgca gtgtgggcac tcaacgcccc tgttccacgc
     4441 accgaatacg cgcggcgtaa gccgctcgca tacatggcgg cgtgcgccga aggccttcgg
     4501 cgcgccgtcg atggcgaccg cagttactca ggcctcatga ccaaaaaccc cggccacatc
     4561 gcctgggaaa cggaatggct ccactcagat ctctacacac tcagccacat cgaggccgag
     4621 ctcggcgcga acatgccacc gccgcgctgg cgtcagcaga ccacgtacaa agcggctccg
     4681 acgccgctag ggcggaattg cgcactgttc gattccgtca ggttgtgggc ctatcttccc
     4741 gccctcatgc ggatctacct gccgacccgg aacgtggacg gactcggccg cgcgatctat
     4801 gccgagtgcc acgcgcgaaa cgccgaattt ccgtgcaacg acgtgtgtcc cggaccgcta
     4861 ccggacagcg aggtccgcgc catcgccaac agcatttggc gttggatcac aaccaagtcg
     4921 cgcatttggg cggacgggat cgtggtctac gaggccacac tcagtgcgcg ccatgcggcc
     4981 atctcgcgga agggcgcagc agcgcgcacg gcggcgagca cagttgcgcg gcgcgcaaag
     5041 tccgcgtcag ccatggaggc attgctatga gcgacggcta cagcgacggc tacagcgacg
     5101 gctacaactg gcagccgact gtccgcaaaa agcggcgcgt gaccgccgcc gaaggcgctc
     5161 gaatcaccgg actatccgaa cgccacgtcg tccggctcgt ggcgcaggaa cgcagcgagt
     5221 ggttcgccga gcaggctgca cgccgcgaac gcatccgcgc ctatcacgac gacgagggcc
     5281 actcttggcc gcaaacggcc aaacatttcg ggctgcatct ggacaccgtt aagcgactcg
     5341 gctatcgggc gaggaaagag cgtgcggcag aacaggaagc ggctcaaaag gcccacaacg
     5401 aagccgacaa tccaccgctg ttctaacgca attggggagc gggtgtcgcg ggggttccgt
     5461 ggggggttcc gttgcaacgg gtcggacagg taaaagtcct ggtagacgct agttttctgg
     5521 tttgggccat gcctgtctcg ttgcgtgttt cgttgcgtcc gttttgaata ccagccagac
     5581 gagacggggt tctacgaatc ttggtcgata ccaagccatt tccgctgaat atcgtggagc
     5641 tcaccgccag aatcggtggt tgtggtgatg tacgtggcga actccgttgt agtgcttgtg
     5701 gtggcatccg tggcgcggcc gctctagaac tagtggatct gacccgctcc acaaccgctg
     5761 agcagggtgg cagtcagagc gaccaggccg accgatacga ggcggatttt gaacaggttc
     5821 actcgaagcc tcccgctggg ttcggagcat cgtcgatcac atgacggcgt gctgacaccg
     5881 attcgactat gcacagtagt aggtagccgc cgcggacggt agcgcaggcg gcctcggcgc
     5941 cgtcgtggcg tcgcgatggt tggcgccaga cccgcctgat cggcgtcccg accagggccg
     6001 ccggggtggt gagcctcggc ttggtccagc cgtagtgaga cgggatgtct tcccggttgt
     6061 cgggtttggg tgggcggtca catgttcgct ggctgtgggc gtgtcggggg tcgtcgaagc
     6121 ggccgcgaac agggctggac gatgcacgtc ttgcacctcc tgtcaacccc caatgttgag
     6181 ccgcgatgcc cctgacctgc accgttggac cgcagtcgtt aggggggcgt gttagcatgg
     6241 agtgacgaaa ggggcctaaa tcagatgatc ccccgggctg caggaattcg atatcaagct
     6301 tatcgatacc gtcgacctcg agggggggcc cggtac
//
