LOCUS       sequence-138621-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pcin/lac-w reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pCH116
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             156..185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    370..386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cacacaacgt cttggagcgc cagaggagga acgagctaaa acggagcttt tttgccctgc
       61 gtgaccagat cccggagttg gaaaacaatg aaaaggcccc caaggtagtt atccttaaaa
      121 aagccacagc atacatcctg tccgtccaag cagaggagca aaagctcatt tctgaagagg
      181 acttgttgcg gaaacgacga gaacagttga aacacaaact tgaacagcta cggaactctt
      241 gtgcgtaagg aaaagtaagg aaaacgattc cttctaacag aaatgtcctg agcaatcacc
      301 tatgaactgt cgagggggcc tatctgaggg aatttacttg tgagcgctca caattgatac
      361 tgagcacaat tgtgagcgga taacaattgc atgccactta aaaaggagat caacaatgaa
      421 agcaattttc gtactgaaac atcttaatca tgctaaggag aaatactagt atgcgtaaag
      481 gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg
      541 gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc
      601 tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg gtaacgacgc
      661 tgacttatgg tgttcagtgc tttgctcgtt atccggacca
//
