LOCUS       sequence-138628-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Prhl/lac-m reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pCH186
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             157..186
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    371..387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 acacacaacg tcttggagcg ccagaggagg aacgagctaa aacggagctt ttttgccctg
       61 cgtgaccaga tcccggagtt ggaaaacaat gaaaaggccc ccaaggtagt tatccttaaa
      121 aaagccacag catacatcct gtccgtccaa gcagaggagc aaaagctcat ttctgaagag
      181 gacttgttgc ggaaacgacg agaacagttg aaacacaaac ttgaacagct acggaactct
      241 tgtgcgtaag gaaaagtaag gaaaacgatt ccttctaaca gaaatgtcct gagcaatcac
      301 ctatgaactg tcgatcctgt gaaatctggc agttttactt gtgagcgctc acaattgata
      361 atgagcacaa ttgtgagcgg ataacaattg catgccactt aaaaaggaga tcaacaatga
      421 aagcaatttt cgtactgaaa catcttaatc atgctaagga gaaatactag tatgcgtaaa
      481 ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg tgatgtcaac
      541 ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg taaactgacg
      601 ctgaagttca tctgtactac tggtaaactg ccggtacctt ggccgactct ggtaacgacg
      661 ctgacttatg gtgttcagtg ctttgctcgt tatccggacc atatgaagca gcatgacttc
      721 ttcaagtccg ccatgccgga aggctatgtg
//
