LOCUS       sequence-13863-e        6336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Takaki K, Davis JM, Winglee K, Ramakrishnan L
  TITLE     Evaluation of the pathogenesis and treatment of Mycobacterium 
            marinum infection in zebrafish.
  JOURNAL   Nat Protoc. 2013 Jun;8(6):1114-24. doi: 10.1038/nprot.2013.068. Epub
            2013 May 16.
  PUBMED    23680983
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.068"; journalName: "Nat Protoc"; date: 
            "2013-06"; volume: "8"; issue: "6"; pages: "1114-24"
COMMENT     Sequence Label: pTEC16
FEATURES             Location/Qualifiers
     source          1..6336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(123..142)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(207..226)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2317..2905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2806..2825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4968..4984
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4968..4984
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5549..5565)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5550..5566)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             5620..6330
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     6033..6052
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cacctgacgt cggggggggg gggaaagcca cgttgtgtct caaaatctct gatgttacat
       61 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
      121 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa
      181 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
      241 aggtgcgaca atctatcgct tgtatgggaa gccccatgcg ccagagttgt ttctgaaaca
      301 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
      361 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg actagagcat
      421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
      481 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
      541 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgacgtggc
      601 cgaccagccc gtcatcgtca acgcctgatc cgcggtgcgg acaggccgtg tcgcgaccgg
      661 ccgtgcggaa ttaagccggc ccgtaccctg tgaatagagg tccgctgtga cacaagaatc
      721 cctgttactt ctcgaccgta ttgattcgga tgattcctac gcgagcctgc ggaacgacca
      781 ggaattctgg gagccgctgg cccgccgagc cctggaggag ctcgggctgc cggtgccgcc
      841 ggtgctgcgg gtgcccggcg agagcaccaa ccccgtactg gtcggcgagc ccggcccggt
      901 gatcaagctg ttcggcgagc actggtgcgg tccggagagc ctcgcgtcgg agtcggaggc
      961 gtacgcggtc ctggcggacg ccccggtgcc ggtgccccgc ctcctcggcc gcggcgagct
     1021 gcggcccggc accggagcct ggccgtggcc ctacctggtg atgagccgga tgaccggcac
     1081 cacctggcgg tccgcgatgg acggcacgac cgaccggaac gcgctgctcg ccctggcccg
     1141 cgaactcggc cgggtgctcg gccggctgca cagggtgccg ctgaccggga acaccgtgct
     1201 caccccccat tccgaggtct tcccggaact gctgcgggaa cgccgcgcgg cgaccgtcga
     1261 ggaccaccgc gggtggggct acctctcgcc ccggctgctg gaccgcctgg aggactggct
     1321 gccggacgtg gacacgctgc tggccggccg cgaaccccgg ttcgtccacg gcgacctgca
     1381 cgggaccaac atcttcgtgg acctggccgc gaccgaggtc accgggatcg tcgacttcac
     1441 cgacgtctat gcgggagact cccgctacag cctggtgcaa ctgcatctca acgccttccg
     1501 gggcgaccgc gagatcctgg ccgcgctgct cgacggggcg cagtggaagc ggaccgagga
     1561 cttcgcccgc gaactgctcg ccttcacctt cctgcacgac ttcgaggtgt tcgaggagac
     1621 cccgctggat ctctccggct tcaccgatcc ggaggaactg gcgcagttcc tctgggggcc
     1681 gccggacacc gcccccggcg cctgacgccc cctctagtaa tcttttgcca ttctcaccgg
     1741 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     1801 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     1861 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     1921 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     1981 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
     2041 acggcggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct
     2101 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac
     2161 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat
     2221 tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcact agttccactg
     2281 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2341 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2401 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2461 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2521 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2581 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2641 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2701 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2761 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2821 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2881 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2941 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3001 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa
     3061 cgcgtgagcc caccagctcc gtaagttcgg gtgctgtgtg gctcgtaccc gcgcattcag
     3121 gcggcagggg gtctaacggg tctaaggcgg cgtgtacggc cgccacagcg gctcttagcg
     3181 gcccggaaac gtcctcgaaa cgacgcatgt gttcctcctg gttggtacag gtggttgggg
     3241 gtgctcggct gtcgctggtg tttcatcatc agggctcgac gggagagcgg gggagtgtgc
     3301 agttgtgggg tggcccctca gcgaaatatc tgacttggag ctcgtgtcgg accatacacc
     3361 ggtgattaat cgtggtttat tatcaagcgt gagccacgtc gccgacgaat ttgagcagct
     3421 ctggctgccg tactggtccc tggcaagcga cgatctgctc gaggggatct accgccaaag
     3481 ccgcgcgtcg gccctaggcc gccggtacat cgaggcgaac ccaacagcgc tggcaaacct
     3541 gctggtcgtg gacgtagacc atccagacgc agcgctccga gcgctcagcg cccgggggtc
     3601 ccatccgctg cccaacgcga tcgtgggcaa tcgcgccaac ggccacgcac acgcagtgtg
     3661 ggcactcaac gcccctgttc cacgcaccga atacgcgcgg cgtaagccgc tcgcatacat
     3721 ggcggcgtgc gccgaaggcc ttcggcgcgc cgtcgatggc gaccgcagtt actcaggcct
     3781 catgaccaaa aaccccggcc acatcgcctg ggaaacggaa tggctccact cagatctcta
     3841 cacactcagc cacatcgagg ccgagctcgg cgcgaacatg ccaccgccgc gctggcgtca
     3901 gcagaccacg tacaaagcgg ctccgacgcc gctagggcgg aattgcgcac tgttcgattc
     3961 cgtcaggttg tgggcctatc ttcccgccct catgcggatc tacctgccga cccggaacgt
     4021 ggacggactc ggccgcgcga tctatgccga gtgccacgcg cgaaacgccg aatttccgtg
     4081 caacgacgtg tgtcccggac cgctaccgga cagcgaggtc cgcgccatcg ccaacagcat
     4141 ttggcgttgg atcacaacca agtcgcgcat ttgggcggac gggatcgtgg tctacgaggc
     4201 cacactcagt gcgcgccatg cggccatctc gcggaagggc gcagcagcgc gcacggcggc
     4261 gagcacagtt gcgcggcgcg caaagtccgc gtcagccatg gaggcattgc tatgagcgac
     4321 ggctacagcg acggctacag cgacggctac aactggcagc cgactgtccg caaaaagcgg
     4381 cgcgtgaccg ccgccgaagg cgctcgaatc accggactat ccgaacgcca cgtcgtccgg
     4441 ctcgtggcgc aggaacgcag cgagtggttc gccgagcagg ctgcacgccg cgaacgcatc
     4501 cgcgcctatc acgacgacga gggccactct tggccgcaaa cggccaaaca tttcgggctg
     4561 catctggaca ccgttaagcg actcggctat cgggcgagga aagagcgtgc ggcagaacag
     4621 gaagcggctc aaaaggccca caacgaagcc gacaatccac cgctgttcta acgcaattgg
     4681 ggagcgggtg tcgcgggggt tccgtggggg gttccgttgc aacgggtcgg acaggtaaaa
     4741 gtcctggtag acgctagttt tctggtttgg gccatgcctg tctcgttgcg tgtttcgttg
     4801 cgtccgtttt gaataccagc cagacgagac ggggttctac gaatcttggt cgataccaag
     4861 ccatttccgc tgaatatcgt ggagctcacc gccagaatcg gtggttgtgg tgatgtacgt
     4921 ggcgaactcc gttgtagtgc ttgtggtggc atccgtggcg cggccgctct agaactagtg
     4981 gatctgaccc gctccacaac cgctgagcag ggtggcagtc agagcgacca ggccgaccga
     5041 tacgaggcgg attttgaaca ggttcactcg aagcctcccg ctgggttcgg agcatcgtcg
     5101 atcacatgac ggcgtgctga caccgattcg actatgcaca gtagtaggta gccgccgcgg
     5161 acggtagcgc aggcggcctc ggcgccgtcg tggcgtcgcg atggttggcg ccagacccgc
     5221 ctgatcggcg tcccgaccag ggccgccggg gtggtgagcc tcggcttggt ccagccgtag
     5281 tgagacggga tgtcttcccg gttgtcgggt ttgggtgggc ggtcacatgt tcgctggctg
     5341 tgggcgtgtc gggggtcgtc gaagcggccg cgaacagggc tggacgatgc acgtcttgca
     5401 cctcctgtca acccccaatg ttgagccgcg atgcccctga cctgcaccgt tggaccgcag
     5461 tcgttagggg ggcgtgttag catggagtga cgaaaggggc ctaaatcaga tgatcccccg
     5521 ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
     5581 ccagatcttt aaatctagat ttaagaagga gatatacata tggtgagcaa gggcgaggag
     5641 aataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg
     5701 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggctttcag
     5761 accgctaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     5821 cctcatttca cctacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     5881 ttcaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaacta cgaggacggc
     5941 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     6001 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     6061 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg caagatcaag
     6121 atgaggctga agctgaagga cggcggccac tacacctccg aggtcaagac cacctacaag
     6181 gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg acatcaagtt ggacatcacc
     6241 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     6301 accggcggca tggacgagct gtacaagtaa gctagc
//
