LOCUS       sequence-138635-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pcin/tet-m reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pCH189
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             157..186
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    340..358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    370..388
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 acacacaacg tcttggagcg ccagaggagg aacgagctaa aacggagctt ttttgccctg
       61 cgtgaccaga tcccggagtt ggaaaacaat gaaaaggccc ccaaggtagt tatccttaaa
      121 aaagccacag catacatcct gtccgtccaa gcagaggagc aaaagctcat ttctgaagag
      181 gacttgttgc ggaaacgacg agaacagttg aaacacaaac ttgaacagct acggaactct
      241 tgtgcgtggc gcatgtcgtt atcgcaaaac cgctgcacac ttttgcgcga catgctctga
      301 tccccctcat ctgggggggc ctatctgagg gaatttactt ccctatcagt gatagagata
      361 atgagcacat ccctatcagt gatagagatg catgccactt aaaaaggaga tcaacaatga
      421 aagcaatttt cgtactgaaa catcttaatc atgctaagga gaaatactag tatgcgtaaa
      481 ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg tgatgtcaac
      541 ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg taaactgacg
      601 ctgaagttca tctgtactac tggtaaactg ccggtacctt ggccgactct ggtaacgacg
      661 ctgacttatg gtgttcagtg ctttgctcgt tatccggacc
//
