LOCUS       sequence-13864-e        6336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Takaki K, Davis JM, Winglee K, Ramakrishnan L
  TITLE     Evaluation of the pathogenesis and treatment of Mycobacterium 
            marinum infection in zebrafish.
  JOURNAL   Nat Protoc. 2013 Jun;8(6):1114-24. doi: 10.1038/nprot.2013.068. Epub
            2013 May 16.
  PUBMED    23680983
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.068"; journalName: "Nat Protoc"; date: 
            "2013-06"; volume: "8"; issue: "6"; pages: "1114-24"
COMMENT     Sequence Label: pTEC17
FEATURES             Location/Qualifiers
     source          1..6336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(32..48)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(33..49)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             103..813
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     primer_bind     complement(942..961)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1026..1045)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3136..3724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3625..3644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5787..5803
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5787..5803
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg
       61 gtaccagatc tttaaatcta gatttaagaa ggagatatac atatggtgag caagggcgag
      121 gagaccacaa tgggcgtaat caagcccgac atgaagatca agctgaagat ggagggcaac
      181 gtgaatggcc acgccttcgt gatcgagggc gagggcgagg gcaagcccta cgacggcacc
      241 aacaccatca acctggaggt gaaggaggga gcccccctgc ccttctccta cgacattctg
      301 accaccgcgt tcgcctacgg caacagggcc ttcaccaagt accccgacga catccccaac
      361 tacttcaagc agtccttccc cgagggctac tcttgggagc gcaccatgac cttcgaggac
      421 aagggcatcg tgaaggtgaa gtccgacatc tccatggagg aggactcctt catctacgag
      481 atacacctca agggcgagaa cttccccccc aacggccccg tgatgcagaa gaagaccacc
      541 ggctgggacg cctccaccga gaggatgtac gtgcgcgacg gcgtgctgaa gggcgacgtc
      601 aagcacaagc tgctgctgga gggcggcggc caccaccgcg ttgacttcaa gaccatctac
      661 agggccaaga aggcggtgaa gctgcccgac tatcactttg tggaccaccg catcgagatc
      721 ctgaaccacg acaaggacta caacaaggtg accgtttacg agagcgccgt ggcccgcaac
      781 tccaccgacg gcatggacga gctgtacaag taagctagcc acctgacgtc gggggggggg
      841 ggaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      901 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      961 tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt
     1021 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     1081 gtatgggaag ccccatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1141 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1201 catcaagcat tttatccgta ctcctgatga ctagagcatg agattatcaa aaaggatctt
     1261 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1321 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1381 atttcgttca tccatagttg cctgactccc cgacgtggcc gaccagcccg tcatcgtcaa
     1441 cgcctgatcc gcggtgcgga caggccgtgt cgcgaccggc cgtgcggaat taagccggcc
     1501 cgtaccctgt gaatagaggt ccgctgtgac acaagaatcc ctgttacttc tcgaccgtat
     1561 tgattcggat gattcctacg cgagcctgcg gaacgaccag gaattctggg agccgctggc
     1621 ccgccgagcc ctggaggagc tcgggctgcc ggtgccgccg gtgctgcggg tgcccggcga
     1681 gagcaccaac cccgtactgg tcggcgagcc cggcccggtg atcaagctgt tcggcgagca
     1741 ctggtgcggt ccggagagcc tcgcgtcgga gtcggaggcg tacgcggtcc tggcggacgc
     1801 cccggtgccg gtgccccgcc tcctcggccg cggcgagctg cggcccggca ccggagcctg
     1861 gccgtggccc tacctggtga tgagccggat gaccggcacc acctggcggt ccgcgatgga
     1921 cggcacgacc gaccggaacg cgctgctcgc cctggcccgc gaactcggcc gggtgctcgg
     1981 ccggctgcac agggtgccgc tgaccgggaa caccgtgctc accccccatt ccgaggtctt
     2041 cccggaactg ctgcgggaac gccgcgcggc gaccgtcgag gaccaccgcg ggtggggcta
     2101 cctctcgccc cggctgctgg accgcctgga ggactggctg ccggacgtgg acacgctgct
     2161 ggccggccgc gaaccccggt tcgtccacgg cgacctgcac gggaccaaca tcttcgtgga
     2221 cctggccgcg accgaggtca ccgggatcgt cgacttcacc gacgtctatg cgggagactc
     2281 ccgctacagc ctggtgcaac tgcatctcaa cgccttccgg ggcgaccgcg agatcctggc
     2341 cgcgctgctc gacggggcgc agtggaagcg gaccgaggac ttcgcccgcg aactgctcgc
     2401 cttcaccttc ctgcacgact tcgaggtgtt cgaggagacc ccgctggatc tctccggctt
     2461 caccgatccg gaggaactgg cgcagttcct ctgggggccg ccggacaccg cccccggcgc
     2521 ctgacgcccc ctctagtaat cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     2581 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     2641 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     2701 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     2761 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     2821 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcggcttt gttgaataaa
     2881 tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgttc
     2941 cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa
     3001 ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcctggt atgagtcagc
     3061 aacaccttct tcacgaggca gacctcacta gttccactga gcgtcagacc ccgtagaaaa
     3121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3241 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
     3541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3841 agctgatacc gctcgccgca gccgaacgac cgagcgcaac gcgtgagccc accagctccg
     3901 taagttcggg tgctgtgtgg ctcgtacccg cgcattcagg cggcaggggg tctaacgggt
     3961 ctaaggcggc gtgtacggcc gccacagcgg ctcttagcgg cccggaaacg tcctcgaaac
     4021 gacgcatgtg ttcctcctgg ttggtacagg tggttggggg tgctcggctg tcgctggtgt
     4081 ttcatcatca gggctcgacg ggagagcggg ggagtgtgca gttgtggggt ggcccctcag
     4141 cgaaatatct gacttggagc tcgtgtcgga ccatacaccg gtgattaatc gtggtttatt
     4201 atcaagcgtg agccacgtcg ccgacgaatt tgagcagctc tggctgccgt actggtccct
     4261 ggcaagcgac gatctgctcg aggggatcta ccgccaaagc cgcgcgtcgg ccctaggccg
     4321 ccggtacatc gaggcgaacc caacagcgct ggcaaacctg ctggtcgtgg acgtagacca
     4381 tccagacgca gcgctccgag cgctcagcgc ccgggggtcc catccgctgc ccaacgcgat
     4441 cgtgggcaat cgcgccaacg gccacgcaca cgcagtgtgg gcactcaacg cccctgttcc
     4501 acgcaccgaa tacgcgcggc gtaagccgct cgcatacatg gcggcgtgcg ccgaaggcct
     4561 tcggcgcgcc gtcgatggcg accgcagtta ctcaggcctc atgaccaaaa accccggcca
     4621 catcgcctgg gaaacggaat ggctccactc agatctctac acactcagcc acatcgaggc
     4681 cgagctcggc gcgaacatgc caccgccgcg ctggcgtcag cagaccacgt acaaagcggc
     4741 tccgacgccg ctagggcgga attgcgcact gttcgattcc gtcaggttgt gggcctatct
     4801 tcccgccctc atgcggatct acctgccgac ccggaacgtg gacggactcg gccgcgcgat
     4861 ctatgccgag tgccacgcgc gaaacgccga atttccgtgc aacgacgtgt gtcccggacc
     4921 gctaccggac agcgaggtcc gcgccatcgc caacagcatt tggcgttgga tcacaaccaa
     4981 gtcgcgcatt tgggcggacg ggatcgtggt ctacgaggcc acactcagtg cgcgccatgc
     5041 ggccatctcg cggaagggcg cagcagcgcg cacggcggcg agcacagttg cgcggcgcgc
     5101 aaagtccgcg tcagccatgg aggcattgct atgagcgacg gctacagcga cggctacagc
     5161 gacggctaca actggcagcc gactgtccgc aaaaagcggc gcgtgaccgc cgccgaaggc
     5221 gctcgaatca ccggactatc cgaacgccac gtcgtccggc tcgtggcgca ggaacgcagc
     5281 gagtggttcg ccgagcaggc tgcacgccgc gaacgcatcc gcgcctatca cgacgacgag
     5341 ggccactctt ggccgcaaac ggccaaacat ttcgggctgc atctggacac cgttaagcga
     5401 ctcggctatc gggcgaggaa agagcgtgcg gcagaacagg aagcggctca aaaggcccac
     5461 aacgaagccg acaatccacc gctgttctaa cgcaattggg gagcgggtgt cgcgggggtt
     5521 ccgtgggggg ttccgttgca acgggtcgga caggtaaaag tcctggtaga cgctagtttt
     5581 ctggtttggg ccatgcctgt ctcgttgcgt gtttcgttgc gtccgttttg aataccagcc
     5641 agacgagacg gggttctacg aatcttggtc gataccaagc catttccgct gaatatcgtg
     5701 gagctcaccg ccagaatcgg tggttgtggt gatgtacgtg gcgaactccg ttgtagtgct
     5761 tgtggtggca tccgtggcgc ggccgctcta gaactagtgg atctgacccg ctccacaacc
     5821 gctgagcagg gtggcagtca gagcgaccag gccgaccgat acgaggcgga ttttgaacag
     5881 gttcactcga agcctcccgc tgggttcgga gcatcgtcga tcacatgacg gcgtgctgac
     5941 accgattcga ctatgcacag tagtaggtag ccgccgcgga cggtagcgca ggcggcctcg
     6001 gcgccgtcgt ggcgtcgcga tggttggcgc cagacccgcc tgatcggcgt cccgaccagg
     6061 gccgccgggg tggtgagcct cggcttggtc cagccgtagt gagacgggat gtcttcccgg
     6121 ttgtcgggtt tgggtgggcg gtcacatgtt cgctggctgt gggcgtgtcg ggggtcgtcg
     6181 aagcggccgc gaacagggct ggacgatgca cgtcttgcac ctcctgtcaa cccccaatgt
     6241 tgagccgcga tgcccctgac ctgcaccgtt ggaccgcagt cgttaggggg gcgtgttagc
     6301 atggagtgac gaaaggggcc taaatcagat gatccc
//
