LOCUS       sequence-138642-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK375
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1044..1066
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattct cagatcgcga gcgtacgttt gatgacgtcg caataggcgt
      121 ccatgaagcg ttttgagagg gcggcgtcag ggacgaacat atcgaagacg tggtaggcgc
      181 cgggcaccac gaagacttcg acgggaacgc cggcagccat caggcgggtg gcgtaatcga
      241 ggtcttcgca tagaaatagg tccatggagc cgaccccgat gaaggtgggc ggcagaccag
      301 cgaggtttgt tgcgcgtccg gcggctgcat agtccggggt tttatcgccg ccagctgcgt
      361 ctccgaggta gcactgccag gcgtatttgt tggctgagcg ggtccagaca tattcaccga
      421 acgcgggggc aggttcgtgt tttatcgccg tgcggtcatc aagcatcggg tagatgagga
      481 tctgacaagc gagtttgtat tcgccgcgat cacgggccat gagggcgaga gccgcgctga
      541 ggccgccgcc cgcgctttct ccggcgacaa cgactttatc gcggttgata cccaagcggg
      601 ctgcgttttc gttcacccat ttgagaactg cgtaacagtc ttcgatggcg ccgggatatt
      661 tcacttccgg agcgagacga taatcgaccg agaagatgtg catgccgaga cggttggcta
      721 ggatctggca tttcggatcg tccacttccg ggtggccgga gatgagaccg ccgccgtgaa
      781 tatagaccat ggcaggagcg ttgggggcga cttttcgggg gctgtagacg aggacacgca
      841 cgtcgggcgc cttgttgagg ccggggacgt attcttcccg tgtatcgaca tctggcgaag
      901 gtctgccttc catgagccgg atagaggtaa ggaagatttc ccgtacttcc ggcagtgaac
      961 gatccgaaag accgtcgaaa gacggcattt ttgccagaac gggcagaaat tccggattta
     1021 cgacattctt tggcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg
     1081 gaaaccgttg tggt
//
