LOCUS       sequence-138643-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pcin/lac-m reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pCH188
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             164..193
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    378..394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gaggcgaaca cacaacgtct tggagcgcca gaggaggaac gagctaaaac ggagcttttt
       61 tgccctgcgt gaccagatcc cggagttgga aaacaatgaa aaggccccca aggtagttat
      121 ccttaaaaaa gccacagcat acatcctgtc cgtccaagca gaggagcaaa agctcatttc
      181 tgaagaggac ttgttgcgga aacgacgaga acagttgaaa cacaaacttg aacagctacg
      241 gaactcttgt gcgtggcgca tgtcgttatc gcaaaaccgc tgcacacttt tgcgcgacat
      301 gctctgatcc ccctcatctg ggggggccta tctgagggaa tttacttgtg agcgctcaca
      361 attgataatg agcacaattg tgagcggata acaattgcat gccacttaaa aaggagatca
      421 acaatgaaag caattttcgt actgaaacat cttaatcatg ctaaggagaa atactagtat
      481 gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac tggatggtga
      541 tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa
      601 actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc cgactctggt
      661 aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccata tgaagcagca
      721 tgactt
//
