LOCUS       sequence-138649-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Prhl/lac-s reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pCH194
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             167..196
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    380..396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcagaggcga acacacaacg tcttggagcg ccagaggagg aacgagctaa aacggagctt
       61 ttttgccctg cgtgaccaga tcccggagtt ggaaaacaat gaaaaggccc ccaaggtagt
      121 tatccttaaa aaagccacag catacatcct gtccgtccaa gcagaggagc aaaagctcat
      181 ttctgaagag gacttgttgc ggaaacgacg agaacagttg aaacacaaac ttgaacagct
      241 acggaactct tgtgcgtaag gaaaagtaag gaaaacgatt ccttctaaca gaaatgtcct
      301 gagcaatcac ctatgaactg tcgatcctgt gaaatctggc agtttacttg tgagcgctca
      361 caattgataa tgagcacaat tgtgagcgga taacaattgc atgccactta aaaaggagat
      421 caacaatgaa agcaattttc gtactgaaac atcttaatca tgctaaggag aaatactagt
      481 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
      541 gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt
      601 aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg
      661 gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca
//
