LOCUS       sequence-138652-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PH domain for phosphoinositide binding 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray 
            D, Emr SD, Lemmon MA
  TITLE     Genome-wide analysis of membrane targeting by S. cerevisiae 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2004 Mar 12;13(5):677-88.
  PUBMED    15023338
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-03-12"; volume: "13(5)"; pages: "677-88"
COMMENT     Sequence Label: pGEX2TK-Num1-PH
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(476..498)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(643..661)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatctcgtcg tgcatctgtt ggatccaacg aaccaagcat aatacccgcg ttaacccaaa
       61 ccgtcattgg ggaatatttg tttaagtatt atccacgctt gggacctttt ggattcgaat
      121 cacgtcatga aagattcttc tgggttcatc catatacctt aactttgtac tggtccgctt
      181 ctaatcccat cctagagaat cctgccaata ccaaaacaaa aggtgttgcc attctaggag
      241 tagaaagtgt cacagaccca aacccatatc caacaggatt gtatcacaaa agtattgttg
      301 ttaccacaga aactaggact attaagttta cttgtcctac aaggcaaaga cacaatattt
      361 ggtataattc attacgttat ttacttcaaa ggaacatgca agggataagt ttagagtgaa
      421 ttcatcgtga ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
      481 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
      541 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
      601 cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg atacgcctat
      661 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttt
//
