LOCUS       sequence-138653-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PH domain for phosphoinositide binding 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray 
            D, Emr SD, Lemmon MA
  TITLE     Genome-wide analysis of membrane targeting by S. cerevisiae 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2004 Mar 12;13(5):677-88.
  PUBMED    15023338
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-03-12"; volume: "13(5)"; pages: "677-88"
COMMENT     Sequence Label: pGEX2TK-Osh2-PH
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(452..474)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(619..637)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatctcgtcg tgcatctgtt ggatcccaag ctattgttca cgaagatgtc gcatcaagta
       61 agcctcctac gtataagggg tttttaaaga aatggacaaa ttttgcccat ggctacaaat
      121 tacgttggtt cattttaagt ggcgacggaa atctgtcata ttacaaagac caatcgcatg
      181 ttgataggcc tcgtggtact ttaaaagttt cgacgtgccg cttacacatc gattcttcag
      241 aaaaactgaa tttcgagttg ctaggtggca tcacaggcac aacaaggtgg cgcttaaagg
      301 gcaatcatcc cattgaaacg actaggtggg tcaatgctat acaaagcgct attagattcg
      361 ccaaagacaa ggagatatta aacaaaaaaa agtgaattca tcgtgactga ctgacgatct
      421 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      481 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      541 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
      601 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      661 taatggtttc ttagacgtca ggtggcactt ttcgg
//
