LOCUS       sequence-138654-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PH domain for phosphoinositide binding 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray 
            D, Emr SD, Lemmon MA
  TITLE     Genome-wide analysis of membrane targeting by S. cerevisiae 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2004 Mar 12;13(5):677-88.
  PUBMED    15023338
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-03-12"; volume: "13(5)"; pages: "677-88"
COMMENT     Sequence Label: pGEX2TK-Skm1-PH
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(482..504)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(649..667)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatctcgtcg tgcatctgtt ggatccatga agggcgtaaa aaaggaagga tggatatctt
       61 ataaagttga tggattgttt tcgttcttat ggcaaaagag atacttggta ctgaatgatt
      121 cgtatttagc attttacaaa agtgataagt gcaatgagga accagtctta tctgtgcctt
      181 tgactagtat aacaaatgtt agcagaatac aattgaaaca aaattgtttt gagattcttc
      241 gggcaacaga tcaaaaagag aacatatccc ccataaactc ctacttttat gaatcaaatt
      301 ccaaaagatc gatattcatt tccacaagaa ccgaacggga tttgcatggc tggcttgatg
      361 ccatttttgc caaatgtcct ctccttagtg gtgtttcatc accaacaaat tttacacaca
      421 aatgaattca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa
      481 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      541 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
      601 cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac
      661 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      721 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      781 atccgctcat gagacaataa ccctgataa
//
