LOCUS       sequence-13866-e        6303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Takaki K, Davis JM, Winglee K, Ramakrishnan L
  TITLE     Evaluation of the pathogenesis and treatment of Mycobacterium 
            marinum infection in zebrafish.
  JOURNAL   Nat Protoc. 2013 Jun;8(6):1114-24. doi: 10.1038/nprot.2013.068. Epub
            2013 May 16.
  PUBMED    23680983
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.068"; journalName: "Nat Protoc"; date: 
            "2013-06"; volume: "8"; issue: "6"; pages: "1114-24"
COMMENT     Sequence Label: pTEC19
FEATURES             Location/Qualifiers
     source          1..6303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..49)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(34..50)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             104..781
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     502..521
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(910..929)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(994..1013)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3104..3692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3593..3612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5755..5771
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5755..5771
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
       61 ggtaccagat ctttaaatct agatttaaga aggagatata catatggata gcactgagaa
      121 cgtcatcaag cccttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      181 gttcgagatc gagggcgtgg gcgagggcaa gccctacgag ggcacccaga ccgccaagct
      241 gcaagtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttctt
      301 ctacggctcc aaggcgtaca tcaagcaccc cgccgacatc cccgactacc tcaagcagtc
      361 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      421 cgtgacccag gactcctccc tgcaggacgg caccctcatc taccacgtga agttcatcgg
      481 cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actctgggct gggagccctc
      541 cactgagcgc aactaccccc gcgacggcgt gctgaagggc gagaaccaca tggcgctgaa
      601 gctgaagggc ggcggccact acctgtgtga gttcaagtcc atctacatgg ccaagaagcc
      661 cgtgaagctg cccggctacc actacgtgga ctacaagctc gacatcacct cccacaacga
      721 ggactacacc gtggtggagc agtacgagcg cgccgaggcc cgccaccacc tgttccagta
      781 ggctagccac ctgacgtcgg gggggggggg aaagccacgt tgtgtctcaa aatctctgat
      841 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
      901 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc
      961 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     1021 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc
     1081 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     1141 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgact
     1201 agagcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1261 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1321 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1381 acgtggccga ccagcccgtc atcgtcaacg cctgatccgc ggtgcggaca ggccgtgtcg
     1441 cgaccggccg tgcggaatta agccggcccg taccctgtga atagaggtcc gctgtgacac
     1501 aagaatccct gttacttctc gaccgtattg attcggatga ttcctacgcg agcctgcgga
     1561 acgaccagga attctgggag ccgctggccc gccgagccct ggaggagctc gggctgccgg
     1621 tgccgccggt gctgcgggtg cccggcgaga gcaccaaccc cgtactggtc ggcgagcccg
     1681 gcccggtgat caagctgttc ggcgagcact ggtgcggtcc ggagagcctc gcgtcggagt
     1741 cggaggcgta cgcggtcctg gcggacgccc cggtgccggt gccccgcctc ctcggccgcg
     1801 gcgagctgcg gcccggcacc ggagcctggc cgtggcccta cctggtgatg agccggatga
     1861 ccggcaccac ctggcggtcc gcgatggacg gcacgaccga ccggaacgcg ctgctcgccc
     1921 tggcccgcga actcggccgg gtgctcggcc ggctgcacag ggtgccgctg accgggaaca
     1981 ccgtgctcac cccccattcc gaggtcttcc cggaactgct gcgggaacgc cgcgcggcga
     2041 ccgtcgagga ccaccgcggg tggggctacc tctcgccccg gctgctggac cgcctggagg
     2101 actggctgcc ggacgtggac acgctgctgg ccggccgcga accccggttc gtccacggcg
     2161 acctgcacgg gaccaacatc ttcgtggacc tggccgcgac cgaggtcacc gggatcgtcg
     2221 acttcaccga cgtctatgcg ggagactccc gctacagcct ggtgcaactg catctcaacg
     2281 ccttccgggg cgaccgcgag atcctggccg cgctgctcga cggggcgcag tggaagcgga
     2341 ccgaggactt cgcccgcgaa ctgctcgcct tcaccttcct gcacgacttc gaggtgttcg
     2401 aggagacccc gctggatctc tccggcttca ccgatccgga ggaactggcg cagttcctct
     2461 gggggccgcc ggacaccgcc cccggcgcct gacgccccct ctagtaatct tttgccattc
     2521 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2581 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2641 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2701 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2761 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     2821 tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca
     2881 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact
     2941 ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg
     3001 gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcactagt
     3061 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3121 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3181 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3241 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3301 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3361 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3421 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3481 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3541 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3601 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3661 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3721 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3781 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3841 agcgcaacgc gtgagcccac cagctccgta agttcgggtg ctgtgtggct cgtacccgcg
     3901 cattcaggcg gcagggggtc taacgggtct aaggcggcgt gtacggccgc cacagcggct
     3961 cttagcggcc cggaaacgtc ctcgaaacga cgcatgtgtt cctcctggtt ggtacaggtg
     4021 gttgggggtg ctcggctgtc gctggtgttt catcatcagg gctcgacggg agagcggggg
     4081 agtgtgcagt tgtggggtgg cccctcagcg aaatatctga cttggagctc gtgtcggacc
     4141 atacaccggt gattaatcgt ggtttattat caagcgtgag ccacgtcgcc gacgaatttg
     4201 agcagctctg gctgccgtac tggtccctgg caagcgacga tctgctcgag gggatctacc
     4261 gccaaagccg cgcgtcggcc ctaggccgcc ggtacatcga ggcgaaccca acagcgctgg
     4321 caaacctgct ggtcgtggac gtagaccatc cagacgcagc gctccgagcg ctcagcgccc
     4381 gggggtccca tccgctgccc aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg
     4441 cagtgtgggc actcaacgcc cctgttccac gcaccgaata cgcgcggcgt aagccgctcg
     4501 catacatggc ggcgtgcgcc gaaggccttc ggcgcgccgt cgatggcgac cgcagttact
     4561 caggcctcat gaccaaaaac cccggccaca tcgcctggga aacggaatgg ctccactcag
     4621 atctctacac actcagccac atcgaggccg agctcggcgc gaacatgcca ccgccgcgct
     4681 ggcgtcagca gaccacgtac aaagcggctc cgacgccgct agggcggaat tgcgcactgt
     4741 tcgattccgt caggttgtgg gcctatcttc ccgccctcat gcggatctac ctgccgaccc
     4801 ggaacgtgga cggactcggc cgcgcgatct atgccgagtg ccacgcgcga aacgccgaat
     4861 ttccgtgcaa cgacgtgtgt cccggaccgc taccggacag cgaggtccgc gccatcgcca
     4921 acagcatttg gcgttggatc acaaccaagt cgcgcatttg ggcggacggg atcgtggtct
     4981 acgaggccac actcagtgcg cgccatgcgg ccatctcgcg gaagggcgca gcagcgcgca
     5041 cggcggcgag cacagttgcg cggcgcgcaa agtccgcgtc agccatggag gcattgctat
     5101 gagcgacggc tacagcgacg gctacagcga cggctacaac tggcagccga ctgtccgcaa
     5161 aaagcggcgc gtgaccgccg ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt
     5221 cgtccggctc gtggcgcagg aacgcagcga gtggttcgcc gagcaggctg cacgccgcga
     5281 acgcatccgc gcctatcacg acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt
     5341 cgggctgcat ctggacaccg ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc
     5401 agaacaggaa gcggctcaaa aggcccacaa cgaagccgac aatccaccgc tgttctaacg
     5461 caattgggga gcgggtgtcg cgggggttcc gtggggggtt ccgttgcaac gggtcggaca
     5521 ggtaaaagtc ctggtagacg ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt
     5581 ttcgttgcgt ccgttttgaa taccagccag acgagacggg gttctacgaa tcttggtcga
     5641 taccaagcca tttccgctga atatcgtgga gctcaccgcc agaatcggtg gttgtggtga
     5701 tgtacgtggc gaactccgtt gtagtgcttg tggtggcatc cgtggcgcgg ccgctctaga
     5761 actagtggat ctgacccgct ccacaaccgc tgagcagggt ggcagtcaga gcgaccaggc
     5821 cgaccgatac gaggcggatt ttgaacaggt tcactcgaag cctcccgctg ggttcggagc
     5881 atcgtcgatc acatgacggc gtgctgacac cgattcgact atgcacagta gtaggtagcc
     5941 gccgcggacg gtagcgcagg cggcctcggc gccgtcgtgg cgtcgcgatg gttggcgcca
     6001 gacccgcctg atcggcgtcc cgaccagggc cgccggggtg gtgagcctcg gcttggtcca
     6061 gccgtagtga gacgggatgt cttcccggtt gtcgggtttg ggtgggcggt cacatgttcg
     6121 ctggctgtgg gcgtgtcggg ggtcgtcgaa gcggccgcga acagggctgg acgatgcacg
     6181 tcttgcacct cctgtcaacc cccaatgttg agccgcgatg cccctgacct gcaccgttgg
     6241 accgcagtcg ttaggggggc gtgttagcat ggagtgacga aaggggccta aatcagatga
     6301 tcc
//
