LOCUS       sequence-13867-e        6324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Takaki K, Davis JM, Winglee K, Ramakrishnan L
  TITLE     Evaluation of the pathogenesis and treatment of Mycobacterium 
            marinum infection in zebrafish.
  JOURNAL   Nat Protoc. 2013 Jun;8(6):1114-24. doi: 10.1038/nprot.2013.068. Epub
            2013 May 16.
  PUBMED    23680983
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.068"; journalName: "Nat Protoc"; date: 
            "2013-06"; volume: "8"; issue: "6"; pages: "1114-24"
COMMENT     Sequence Label: pTEC20
FEATURES             Location/Qualifiers
     source          1..6324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(34..50)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(35..51)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             105..803
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     primer_bind     complement(932..951)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1016..1035)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3126..3714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3615..3634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5777..5793
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5777..5793
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
       61 cggtaccaga tctttaaatc tagatttaag aaggagatat acatatggtg agcgagctga
      121 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
      181 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
      241 cggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
      301 gcagcaaaac cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc
      361 ccgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
      421 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
      481 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gcctccaccg
      541 agaccctgta ccccgctgac ggcggcctgg aaggcagagc cgacatggcc ctgaagctcg
      601 tgggcggggg ccacctgatc tgcaacttga agaccacata cagatccaag aaacccgcta
      661 agaacctcaa gatgcccggc gtctactatg tggacagaag actggaaaga atcaaggagg
      721 ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc
      781 ctagcaaact ggggcacaga tgagctagcc acctgacgtc gggggggggg ggaaagccac
      841 gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
      901 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
      961 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     1021 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     1081 ccccatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     1141 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     1201 tttatccgta ctcctgatga ctagagcatg agattatcaa aaaggatctt cacctagatc
     1261 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1321 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1381 tccatagttg cctgactccc cgacgtggcc gaccagcccg tcatcgtcaa cgcctgatcc
     1441 gcggtgcgga caggccgtgt cgcgaccggc cgtgcggaat taagccggcc cgtaccctgt
     1501 gaatagaggt ccgctgtgac acaagaatcc ctgttacttc tcgaccgtat tgattcggat
     1561 gattcctacg cgagcctgcg gaacgaccag gaattctggg agccgctggc ccgccgagcc
     1621 ctggaggagc tcgggctgcc ggtgccgccg gtgctgcggg tgcccggcga gagcaccaac
     1681 cccgtactgg tcggcgagcc cggcccggtg atcaagctgt tcggcgagca ctggtgcggt
     1741 ccggagagcc tcgcgtcgga gtcggaggcg tacgcggtcc tggcggacgc cccggtgccg
     1801 gtgccccgcc tcctcggccg cggcgagctg cggcccggca ccggagcctg gccgtggccc
     1861 tacctggtga tgagccggat gaccggcacc acctggcggt ccgcgatgga cggcacgacc
     1921 gaccggaacg cgctgctcgc cctggcccgc gaactcggcc gggtgctcgg ccggctgcac
     1981 agggtgccgc tgaccgggaa caccgtgctc accccccatt ccgaggtctt cccggaactg
     2041 ctgcgggaac gccgcgcggc gaccgtcgag gaccaccgcg ggtggggcta cctctcgccc
     2101 cggctgctgg accgcctgga ggactggctg ccggacgtgg acacgctgct ggccggccgc
     2161 gaaccccggt tcgtccacgg cgacctgcac gggaccaaca tcttcgtgga cctggccgcg
     2221 accgaggtca ccgggatcgt cgacttcacc gacgtctatg cgggagactc ccgctacagc
     2281 ctggtgcaac tgcatctcaa cgccttccgg ggcgaccgcg agatcctggc cgcgctgctc
     2341 gacggggcgc agtggaagcg gaccgaggac ttcgcccgcg aactgctcgc cttcaccttc
     2401 ctgcacgact tcgaggtgtt cgaggagacc ccgctggatc tctccggctt caccgatccg
     2461 gaggaactgg cgcagttcct ctgggggccg ccggacaccg cccccggcgc ctgacgcccc
     2521 ctctagtaat cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2581 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     2641 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     2701 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     2761 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     2821 ctggcagagc attacgctga cttgacggga cggcggcttt gttgaataaa tcgaactttt
     2881 gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgttc cgtggcaaag
     2941 caaaagttca aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc
     3001 ctcactttct ggctggatga tggggcgatt caggcctggt atgagtcagc aacaccttct
     3061 tcacgaggca gacctcacta gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3241 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3301 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3361 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3421 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3481 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     3541 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3601 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3661 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3721 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3781 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3841 gctcgccgca gccgaacgac cgagcgcaac gcgtgagccc accagctccg taagttcggg
     3901 tgctgtgtgg ctcgtacccg cgcattcagg cggcaggggg tctaacgggt ctaaggcggc
     3961 gtgtacggcc gccacagcgg ctcttagcgg cccggaaacg tcctcgaaac gacgcatgtg
     4021 ttcctcctgg ttggtacagg tggttggggg tgctcggctg tcgctggtgt ttcatcatca
     4081 gggctcgacg ggagagcggg ggagtgtgca gttgtggggt ggcccctcag cgaaatatct
     4141 gacttggagc tcgtgtcgga ccatacaccg gtgattaatc gtggtttatt atcaagcgtg
     4201 agccacgtcg ccgacgaatt tgagcagctc tggctgccgt actggtccct ggcaagcgac
     4261 gatctgctcg aggggatcta ccgccaaagc cgcgcgtcgg ccctaggccg ccggtacatc
     4321 gaggcgaacc caacagcgct ggcaaacctg ctggtcgtgg acgtagacca tccagacgca
     4381 gcgctccgag cgctcagcgc ccgggggtcc catccgctgc ccaacgcgat cgtgggcaat
     4441 cgcgccaacg gccacgcaca cgcagtgtgg gcactcaacg cccctgttcc acgcaccgaa
     4501 tacgcgcggc gtaagccgct cgcatacatg gcggcgtgcg ccgaaggcct tcggcgcgcc
     4561 gtcgatggcg accgcagtta ctcaggcctc atgaccaaaa accccggcca catcgcctgg
     4621 gaaacggaat ggctccactc agatctctac acactcagcc acatcgaggc cgagctcggc
     4681 gcgaacatgc caccgccgcg ctggcgtcag cagaccacgt acaaagcggc tccgacgccg
     4741 ctagggcgga attgcgcact gttcgattcc gtcaggttgt gggcctatct tcccgccctc
     4801 atgcggatct acctgccgac ccggaacgtg gacggactcg gccgcgcgat ctatgccgag
     4861 tgccacgcgc gaaacgccga atttccgtgc aacgacgtgt gtcccggacc gctaccggac
     4921 agcgaggtcc gcgccatcgc caacagcatt tggcgttgga tcacaaccaa gtcgcgcatt
     4981 tgggcggacg ggatcgtggt ctacgaggcc acactcagtg cgcgccatgc ggccatctcg
     5041 cggaagggcg cagcagcgcg cacggcggcg agcacagttg cgcggcgcgc aaagtccgcg
     5101 tcagccatgg aggcattgct atgagcgacg gctacagcga cggctacagc gacggctaca
     5161 actggcagcc gactgtccgc aaaaagcggc gcgtgaccgc cgccgaaggc gctcgaatca
     5221 ccggactatc cgaacgccac gtcgtccggc tcgtggcgca ggaacgcagc gagtggttcg
     5281 ccgagcaggc tgcacgccgc gaacgcatcc gcgcctatca cgacgacgag ggccactctt
     5341 ggccgcaaac ggccaaacat ttcgggctgc atctggacac cgttaagcga ctcggctatc
     5401 gggcgaggaa agagcgtgcg gcagaacagg aagcggctca aaaggcccac aacgaagccg
     5461 acaatccacc gctgttctaa cgcaattggg gagcgggtgt cgcgggggtt ccgtgggggg
     5521 ttccgttgca acgggtcgga caggtaaaag tcctggtaga cgctagtttt ctggtttggg
     5581 ccatgcctgt ctcgttgcgt gtttcgttgc gtccgttttg aataccagcc agacgagacg
     5641 gggttctacg aatcttggtc gataccaagc catttccgct gaatatcgtg gagctcaccg
     5701 ccagaatcgg tggttgtggt gatgtacgtg gcgaactccg ttgtagtgct tgtggtggca
     5761 tccgtggcgc ggccgctcta gaactagtgg atctgacccg ctccacaacc gctgagcagg
     5821 gtggcagtca gagcgaccag gccgaccgat acgaggcgga ttttgaacag gttcactcga
     5881 agcctcccgc tgggttcgga gcatcgtcga tcacatgacg gcgtgctgac accgattcga
     5941 ctatgcacag tagtaggtag ccgccgcgga cggtagcgca ggcggcctcg gcgccgtcgt
     6001 ggcgtcgcga tggttggcgc cagacccgcc tgatcggcgt cccgaccagg gccgccgggg
     6061 tggtgagcct cggcttggtc cagccgtagt gagacgggat gtcttcccgg ttgtcgggtt
     6121 tgggtgggcg gtcacatgtt cgctggctgt gggcgtgtcg ggggtcgtcg aagcggccgc
     6181 gaacagggct ggacgatgca cgtcttgcac ctcctgtcaa cccccaatgt tgagccgcga
     6241 tgcccctgac ctgcaccgtt ggaccgcagt cgttaggggg gcgtgttagc atggagtgac
     6301 gaaaggggcc taaatcagat gatc
//
