LOCUS       sequence-138674-        7424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3D protein (truncated at cleavage site P1; ie, 
            short), C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3d(S)-Fc-His
FEATURES             Location/Qualifiers
     source          1..7424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             4004..4021
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4047..4168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4084..4103)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4138..4157
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4206..4223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4377..4965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4457..4476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5136..5927,5928..5996))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5759..5778
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5997..6101)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6169..6187
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6225..6247)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6249..6269)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6254..6584
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6435..6570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6497..6516
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6721..6740
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6807..7262)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6944..6965)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7156..7175
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7388..7410
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7402..7419
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7403..7419
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgaatgttac
     1621 taaagatgag aacccaagat ccagaagtca agatcttcac ctttttcatg cttggatgat
     1681 gttaatcatg acggtgctct ttcttcctgt cactgaaacg tctaaacaaa atattccaag
     1741 actcaagcta acctacaaag acttgctgct ttcaaacacc tgtatcccct ttttgggttc
     1801 atcagaagga ttggatttcc agactcttct tttggatgag gagaggggca tactgctcct
     1861 aggagccaaa gaccatgtct tcctgctcag tctggttgac ttgaacaaga attttaagaa
     1921 gatttattgg cctgctgcaa aagaacgagt ggagctatgt aaattagctg ggaaagatgc
     1981 caatacagaa tgtgcaaatt tcatccgtgt gcttcaaccc tataataaga ctcacgttta
     2041 cgtgtgtgga actggagcgt ttcatccgct gtgtgggtac atcgatctcg gcgccaacaa
     2101 ggaggaactc atatttaaac tagacacgca caacctggag tctggcagac tgaaatgtcc
     2161 ctttgatcct cagcagcctt ttgcttcagt aatgacagat gagcacctct actctggaac
     2221 agcttctgat ttccttggca aagacactgc attcacaagg tctctggggc taatgcagga
     2281 ccaccattcc atcagaactg acatttcaga gcaccactgg ctcaatggag caaaatttat
     2341 cggaacattc cccattccag acacctataa tccagatgat gataaaatat atttcttctt
     2401 tcgagaatca tcccaggaag gcagtacttc tgacagaagc attctttcaa gagttggaag
     2461 agtttgtaag aatgatgtag gtgggcaacg aagtctgata aacaaatgga caacttttct
     2521 aaaggcaaga ctgatttgct cgattcctgg aagcgatggg gcagataccc attttgatga
     2581 actccaagat atttacttac tccctacgag agatgaaaga aatcctgtag tatatggagt
     2641 ctttaccaca accagctcca tcttcaaagg ctctgctgtc tgtgtgtaca gcatggctga
     2701 tatccgagca gtctttaatg gtccctatgc tcataaggaa agtgctgacc atcgctgggt
     2761 gcaatatgat ggaaggatac cttacccccg acctggaacg tgtccaagca aaacctatga
     2821 cccactgatt aagtccaccc gagactttcc agacgatgtt atcagtttca taaggcggca
     2881 ccctgtgatg tataagtccg tgtacccagt ggcaggagca ccgaccttca agagaatcaa
     2941 cgtggattac agactgacgc agatagtggt ggatcacgtg gtcgctgaag acgggcagta
     3001 tgatgtcatg tttctcggaa cagacattgg aacagtcctg aaagttgtga gcatctccaa
     3061 ggagaagtgg aatatggaag aggtcgtact ggaggagctt caggtattca agcacccaac
     3121 agctatcttg aacatggagt tgtcgctgaa gcagcaacag ttgtacgttg gttcctggga
     3181 tggattggtc cagctctcct tgcacagatg cgacacttac gggaaagcat gtgcagactg
     3241 ctgtctcgcc agagaccctt actgtgcctg ggatggaaat gcttgctcca gatatgcacc
     3301 cacgtctggc gcgcctgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct
     3361 ggggggaccg tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg
     3421 gacccctgag gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt
     3481 caactggtac gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca
     3541 gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa
     3601 tggcaaggag tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac
     3661 catctccaaa gccaaagggc agccccgaga accacaggtg tataccctgc ccccatcccg
     3721 ggatgagctg accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag
     3781 cgacatcgcc gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc
     3841 tcccgtgctg gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag
     3901 caggtggcag caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca
     3961 ctacacgcag aagagcctct ccctgtctcc gggtaaaggc ggccatcatc accatcacca
     4021 ttaatctaga gatcttatta aagcagaact tgtttattgc agcttataat ggttacaaat
     4081 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4141 gtttgtccaa actcatcaat gtatcttatc atgtctggtc gactctagct agactcttcc
     4201 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4261 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4321 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4381 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4441 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4501 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4561 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4621 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4681 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4741 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4801 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4861 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4921 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4981 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5041 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5101 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5161 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5221 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5281 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5341 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5401 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5461 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5521 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5581 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5641 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5701 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5761 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5821 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5881 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5941 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6001 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6061 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6121 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     6181 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     6241 ctcccggagg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca
     6301 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6361 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6421 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6481 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     6541 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     6601 gtatatccat tttcgtcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     6661 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     6721 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     6781 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc
     6841 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     6901 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     6961 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     7021 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     7081 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     7141 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     7201 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     7261 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     7321 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     7381 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aatt
//
