LOCUS       sequence-13868-e        6307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6307)
  TITLE     Ramakrishnan Lab Plasmids
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTEC21
FEATURES             Location/Qualifiers
     source          1..6307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(128..147)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(212..231)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2322..2910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2811..2830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4973..4989
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4973..4989
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5554..5570)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5555..5571)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             5624..6304
                     /codon_start=1
                     /product="Kaede green-to-red photoconvertible fluorescent 
                     protein (Ando et al., 2002)"
                     /label=Kaede
                     /translation="MVSLIKPEMKIKLLMEGNVNGHQFVIEGDGKGHPFEGKQSMDLVV
                     KEGAPLPFAYDILTTAFHYGNRVFAKYPDHIPDYFKQSFPKGFSWERSLMFEDGGVCIA
                     TNDITLKGDTFFNKVRFDGVNFPPNGPVMQKKTLKWEASTEKMYLRDGVLTGDITMALL
                     LKGDVHYRCDFRTTYKSRQEGVKLPGYHFVDHCISILRHDKDYNEVKLYEHAVAHSGLP
                     DNVK"
ORIGIN
        1 ctagccacct gacgtcgggg ggggggggaa agccacgttg tgtctcaaaa tctctgatgt
       61 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
      121 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc gaggccgcga
      181 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      241 caatcaggtg cgacaatcta tcgcttgtat gggaagcccc atgcgccaga gttgtttctg
      301 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
      361 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgactag
      421 agcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgac
      601 gtggccgacc agcccgtcat cgtcaacgcc tgatccgcgg tgcggacagg ccgtgtcgcg
      661 accggccgtg cggaattaag ccggcccgta ccctgtgaat agaggtccgc tgtgacacaa
      721 gaatccctgt tacttctcga ccgtattgat tcggatgatt cctacgcgag cctgcggaac
      781 gaccaggaat tctgggagcc gctggcccgc cgagccctgg aggagctcgg gctgccggtg
      841 ccgccggtgc tgcgggtgcc cggcgagagc accaaccccg tactggtcgg cgagcccggc
      901 ccggtgatca agctgttcgg cgagcactgg tgcggtccgg agagcctcgc gtcggagtcg
      961 gaggcgtacg cggtcctggc ggacgccccg gtgccggtgc cccgcctcct cggccgcggc
     1021 gagctgcggc ccggcaccgg agcctggccg tggccctacc tggtgatgag ccggatgacc
     1081 ggcaccacct ggcggtccgc gatggacggc acgaccgacc ggaacgcgct gctcgccctg
     1141 gcccgcgaac tcggccgggt gctcggccgg ctgcacaggg tgccgctgac cgggaacacc
     1201 gtgctcaccc cccattccga ggtcttcccg gaactgctgc gggaacgccg cgcggcgacc
     1261 gtcgaggacc accgcgggtg gggctacctc tcgccccggc tgctggaccg cctggaggac
     1321 tggctgccgg acgtggacac gctgctggcc ggccgcgaac cccggttcgt ccacggcgac
     1381 ctgcacggga ccaacatctt cgtggacctg gccgcgaccg aggtcaccgg gatcgtcgac
     1441 ttcaccgacg tctatgcggg agactcccgc tacagcctgg tgcaactgca tctcaacgcc
     1501 ttccggggcg accgcgagat cctggccgcg ctgctcgacg gggcgcagtg gaagcggacc
     1561 gaggacttcg cccgcgaact gctcgccttc accttcctgc acgacttcga ggtgttcgag
     1621 gagaccccgc tggatctctc cggcttcacc gatccggagg aactggcgca gttcctctgg
     1681 gggccgccgg acaccgcccc cggcgcctga cgccccctct agtaatcttt tgccattctc
     1741 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     1801 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     1861 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     1921 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     1981 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     2041 acgggacggc ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg
     2101 catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg
     2161 tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg
     2221 gcgattcagg cctggtatga gtcagcaaca ccttcttcac gaggcagacc tcactagttc
     2281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2461 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2701 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2941 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3001 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3061 cgcaacgcgt gagcccacca gctccgtaag ttcgggtgct gtgtggctcg tacccgcgca
     3121 ttcaggcggc agggggtcta acgggtctaa ggcggcgtgt acggccgcca cagcggctct
     3181 tagcggcccg gaaacgtcct cgaaacgacg catgtgttcc tcctggttgg tacaggtggt
     3241 tgggggtgct cggctgtcgc tggtgtttca tcatcagggc tcgacgggag agcgggggag
     3301 tgtgcagttg tggggtggcc cctcagcgaa atatctgact tggagctcgt gtcggaccat
     3361 acaccggtga ttaatcgtgg tttattatca agcgtgagcc acgtcgccga cgaatttgag
     3421 cagctctggc tgccgtactg gtccctggca agcgacgatc tgctcgaggg gatctaccgc
     3481 caaagccgcg cgtcggccct aggccgccgg tacatcgagg cgaacccaac agcgctggca
     3541 aacctgctgg tcgtggacgt agaccatcca gacgcagcgc tccgagcgct cagcgcccgg
     3601 gggtcccatc cgctgcccaa cgcgatcgtg ggcaatcgcg ccaacggcca cgcacacgca
     3661 gtgtgggcac tcaacgcccc tgttccacgc accgaatacg cgcggcgtaa gccgctcgca
     3721 tacatggcgg cgtgcgccga aggccttcgg cgcgccgtcg atggcgaccg cagttactca
     3781 ggcctcatga ccaaaaaccc cggccacatc gcctgggaaa cggaatggct ccactcagat
     3841 ctctacacac tcagccacat cgaggccgag ctcggcgcga acatgccacc gccgcgctgg
     3901 cgtcagcaga ccacgtacaa agcggctccg acgccgctag ggcggaattg cgcactgttc
     3961 gattccgtca ggttgtgggc ctatcttccc gccctcatgc ggatctacct gccgacccgg
     4021 aacgtggacg gactcggccg cgcgatctat gccgagtgcc acgcgcgaaa cgccgaattt
     4081 ccgtgcaacg acgtgtgtcc cggaccgcta ccggacagcg aggtccgcgc catcgccaac
     4141 agcatttggc gttggatcac aaccaagtcg cgcatttggg cggacgggat cgtggtctac
     4201 gaggccacac tcagtgcgcg ccatgcggcc atctcgcgga agggcgcagc agcgcgcacg
     4261 gcggcgagca cagttgcgcg gcgcgcaaag tccgcgtcag ccatggaggc attgctatga
     4321 gcgacggcta cagcgacggc tacagcgacg gctacaactg gcagccgact gtccgcaaaa
     4381 agcggcgcgt gaccgccgcc gaaggcgctc gaatcaccgg actatccgaa cgccacgtcg
     4441 tccggctcgt ggcgcaggaa cgcagcgagt ggttcgccga gcaggctgca cgccgcgaac
     4501 gcatccgcgc ctatcacgac gacgagggcc actcttggcc gcaaacggcc aaacatttcg
     4561 ggctgcatct ggacaccgtt aagcgactcg gctatcgggc gaggaaagag cgtgcggcag
     4621 aacaggaagc ggctcaaaag gcccacaacg aagccgacaa tccaccgctg ttctaacgca
     4681 attggggagc gggtgtcgcg ggggttccgt ggggggttcc gttgcaacgg gtcggacagg
     4741 taaaagtcct ggtagacgct agttttctgg tttgggccat gcctgtctcg ttgcgtgttt
     4801 cgttgcgtcc gttttgaata ccagccagac gagacggggt tctacgaatc ttggtcgata
     4861 ccaagccatt tccgctgaat atcgtggagc tcaccgccag aatcggtggt tgtggtgatg
     4921 tacgtggcga actccgttgt agtgcttgtg gtggcatccg tggcgcggcc gctctagaac
     4981 tagtggatct gacccgctcc acaaccgctg agcagggtgg cagtcagagc gaccaggccg
     5041 accgatacga ggcggatttt gaacaggttc actcgaagcc tcccgctggg ttcggagcat
     5101 cgtcgatcac atgacggcgt gctgacaccg attcgactat gcacagtagt aggtagccgc
     5161 cgcggacggt agcgcaggcg gcctcggcgc cgtcgtggcg tcgcgatggt tggcgccaga
     5221 cccgcctgat cggcgtcccg accagggccg ccggggtggt gagcctcggc ttggtccagc
     5281 cgtagtgaga cgggatgtct tcccggttgt cgggtttggg tgggcggtca catgttcgct
     5341 ggctgtgggc gtgtcggggg tcgtcgaagc ggccgcgaac agggctggac gatgcacgtc
     5401 ttgcacctcc tgtcaacccc caatgttgag ccgcgatgcc cctgacctgc accgttggac
     5461 cgcagtcgtt aggggggcgt gttagcatgg agtgacgaaa ggggcctaaa tcagatgatc
     5521 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
     5581 cggtaccaga tctttaaatc tagatttaga aggagatata catatggtga gtctgattaa
     5641 accagaaatg aagatcaagc tgcttatgga aggcaatgta aacgggcacc agtttgttat
     5701 tgagggagat ggaaaaggcc atccttttga gggaaaacag agtatggacc ttgtagtcaa
     5761 agaaggcgca cctctccctt ttgcctacga tatcttgaca acagcattcc attatggtaa
     5821 cagggttttt gctaaatacc cagaccatat accagactac ttcaagcagt cgtttcccaa
     5881 agggttttct tgggagcgaa gcctgatgtt cgaggacggg ggcgtttgca tcgctacaaa
     5941 tgacataaca ctgaaaggag acactttttt taacaaagtt cgatttgatg gcgtaaactt
     6001 tcccccaaat ggtcctgtta tgcagaagaa gactctgaaa tgggaggcat ccactgagaa
     6061 aatgtatttg cgtgatggag tgttgacggg cgatattaca atggctctgc tgcttaaagg
     6121 agatgtccat taccgatgtg acttcagaac tacttacaaa tctaggcagg agggtgtcaa
     6181 gttgccagga tatcactttg tcgatcactg catcagcata ttgaggcatg acaaagacta
     6241 caacgaggtt aagctgtatg agcacgctgt tgcccattct ggattgccgg acaacgtcaa
     6301 gtaactg
//
