LOCUS       sequence-138683-        7370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3B protein (truncated at cleavage site P1; ie, 
            short), C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3b(S)-Fc-His
FEATURES             Location/Qualifiers
     source          1..7370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3950..3967
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3993..4114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4030..4049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4084..4103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4152..4169)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4323..4911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4403..4422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5082..5873,5874..5942))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5705..5724
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5943..6047)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6115..6133
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6171..6193)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6195..6215)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6200..6530
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6381..6516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6443..6462
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6667..6686
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6753..7208)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6890..6911)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7102..7121
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7334..7356
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7348..7365
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7349..7365
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggggcgggc
     1621 tgaggccgcc gccatgatcc caggcctggc ccttctctgg gtagcagggc taggggatac
     1681 tgcccctaac cttccccgcc ttcggctctc ctttcaagaa ttacaggccc ggcatggtgt
     1741 ccgaaccttc aggctggagc ggacctgctg ttatgaagcc ttgctggtgg atgaggagcg
     1801 tggacgcctg tttgtgggtg ctgagaacca cgtggcttcc ctcagcctgg acaacatcag
     1861 caagcgagcc aagaagttgg cctggcccgc ccccgtggaa tggcgtgaag aatgcaactg
     1921 ggcagggaag gacattggta ccgagtgcat gaacttcatg aagctgctgc acgcctacaa
     1981 ccacacccac ttgctggcct gtggcacagg ggctttccac ccaacctgcg cctttgtgga
     2041 ggtgggccac cggctggagg aacccatgct tcaactggac cggaggaaac ttgaggacgg
     2101 caaggggaag actccttatg acccaaggca tcgggctgcc tcggtgctgg tgggggaaga
     2161 gctgtattct ggggtggcag cagaccttat gggccgggac tttaccatct ttcgaagcct
     2221 tggtcagaat ccgagtctcc gaacagagcc ccatgattcc cgctggctca atgaacccaa
     2281 gtttgtcaag gtcttttgga tcccagagag tgagaaccct gatgacgata aaatctattt
     2341 cttcttccgc gagtccgctg tggaagcagc accagcaatg gggcgcatgt ctgtgtctcg
     2401 tgttggccag atctgcagga atgacctggg tggccagcgg agcttggtca acaaatggac
     2461 cacatttctg aaggcgcggc ttgtgtgctc agtacctgga gttgagggtg acacccactt
     2521 tgaccaactt caggatgttt tccttctgtc ctcccgagac cgccagacac ctcttctcta
     2581 tgctgtcttc tccacctcca gtggtgtctt ccagggctct gctgtgtgcg tgtacagcat
     2641 gaacgatgtg cgccgagcct tcttgggacc ttttgctcac aaagaggggc ctacacacca
     2701 gtgggtgtcc taccagggtc gtgtccccta cccaagacct ggcatgtgcc ccagcaagac
     2761 ctttggcacc ttcagctcca ccaaggactt cccagatgac gttatccagt ttgctcggaa
     2821 ccaccctctc atgtacaacc cagtcctgcc catggggggg cgccctctct tcctacaagt
     2881 gggagctggg tacaccttca cccaaatcgc cgcagaccga gtagcagctg ccgatggaca
     2941 ctacgatgtt ctcttcattg gtacagatgt gggcacagtg ctgaaagtga tctcagtccc
     3001 caaaggcagc cgacctaatt ctgaaggact tctcctggaa gagctgcagg tgttcgagga
     3061 ctctgccgct atcaccagca tgcaaatctc ctctaaaagg caacaactct acgtagcatc
     3121 gcgcagcgca gtggcccaga ttgctttgca tcgctgcact gccctaggcc gcgcctgcgc
     3181 agaatgctgc ttggcccgtg atccttactg cgcctgggat ggatcagctt gcacacgctt
     3241 ccagcctacg gccggcgcgc ctgacaaaac tcacacatgc ccaccgtgcc cagcacctga
     3301 actcctgggg ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat
     3361 ctcccggacc cctgaggtca catgcgtggt ggtggacgtg agccacgaag accctgaggt
     3421 caagttcaac tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccgcggga
     3481 ggagcagtac aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg
     3541 gctgaatggc aaggagtaca agtgcaaggt ctccaacaaa gccctcccag cccccatcga
     3601 gaaaaccatc tccaaagcca aagggcagcc ccgagaacca caggtgtata ccctgccccc
     3661 atcccgggat gagctgacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta
     3721 tcccagcgac atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac
     3781 cacgcctccc gtgctggact ccgacggctc cttcttcctc tacagcaagc tcaccgtgga
     3841 caagagcagg tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg aggctctgca
     3901 caaccactac acgcagaaga gcctctccct gtctccgggt aaaggcggcc atcatcacca
     3961 tcaccattaa tctagagatc ttattaaagc agaacttgtt tattgcagct tataatggtt
     4021 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4081 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggtcgact ctagctagac
     4141 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4201 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4261 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4321 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4501 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4741 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4921 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4981 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5041 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5101 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5161 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5221 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5281 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5341 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5401 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5461 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5521 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5581 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5641 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5701 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5761 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5821 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5881 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5941 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6001 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6061 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     6121 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     6181 atgcagctcc cggaggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg
     6241 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6301 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6361 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6421 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     6481 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     6541 gagcttgtat atccattttc gtcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     6601 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     6661 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     6721 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
     6781 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     6841 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     6901 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     6961 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     7021 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     7081 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     7141 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     7201 gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     7261 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     7321 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
//
