LOCUS       sequence-138685-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For analysis of PH domain localization in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray 
            D, Emr SD, Lemmon MA
  TITLE     Genome-wide analysis of membrane targeting by S. cerevisiae 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2004 Mar 12;13(5):677-88.
  PUBMED    15023338
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-03-12"; volume: "13(5)"; pages: "677-88"
COMMENT     Sequence Label: pRS426-GFP-Osh1-PH
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..20
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5..21
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(464..484)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccctcgaggt cgacggtatc gataagcttg atatcgaatt cagtgcagca aaatttctct
       61 gtcctttgcg tatcttatgg cgccctggat ggcccaaacc catctatttg tctcgatggg
      121 gtggttcccc tttaaatgcc acctgataac accgttgtta ccgccgataa tttcgaattt
      181 caacttttca gacgaatcca aatgcagaga gcacgaagac atgtttaggg agcccctgca
      241 ggcattctta gtgtcggcct gatcgatgta gtaggatagt ttcccatcgc tactaaggat
      301 gaaccaacgc aatttgtagc cttgagcgaa gttggtccat tttttcaggt agcctttgta
      361 agtgggggcc tcgtgcaagt tgttgtttgt gacgtcgatg acactttggg atctgggtcc
      421 ggacttgtat agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag
      481 gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta
      541 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
      601 tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc
      661 attcttttgt ttgtctgcca tgatgtatac attgtgtgag ttatagttgt attccaattt
      721 gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac
      781 aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa
      841 a
//
