LOCUS       sequence-13869-e        7250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Takaki K, Cosma CL, Troll MA, Ramakrishnan L
  TITLE     An in vivo platform for rapid high-throughput antitubercular drug 
            discovery.
  JOURNAL   Cell Rep. 2012 Jul 26;2(1):175-84. doi: 
            10.1016/j.celrep.2012.06.008. Epub 2012 Jul 20.
  PUBMED    22840407
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2012.06.008"; journalName: "Cell Rep"; date: 
            "2012-07-26- 26"; volume: "2"; issue: "1"; pages: "175-84"
COMMENT     Sequence Label: pTEC22
FEATURES             Location/Qualifiers
     source          1..7250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             44..748
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             44..748
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             818..1522
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             818..1522
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     terminator      1570..1664
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1797..1816)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1881..1900)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3991..4579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4480..4499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6642..6658
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6642..6658
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7223..7239)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7224..7240)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ggtaccagat ctttaaatct agatttaaga aggagatata catatggtgg gtgaggatag
       61 cgtgctgatc accgagaaca tgcacatgaa actgtacatg gagggcaccg tgaacgacca
      121 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgaa
      181 gatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
      241 catgtacggc agcaaaacct ttatcaacca cacccagggc atccccgact tctttaagca
      301 gtccttccct gagggcttca catgggagag gatcaccaca tacgaagacg ggggcgtgct
      361 gaccgctacc caggacacca gcctccagaa cggctgcctc atctacaacg tcaagatcaa
      421 cggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
      481 cagcaccgag atgctgtacc ccgctgacag cggcctgaga ggccatgccc agatggccct
      541 gaagctcgtg ggcgggggct acctgcactg ctccctcaag accacataca gatccaagaa
      601 acccgctaag aacctcaaga tgcccggctt ctacttcgtg gacaggagac tggaaagaat
      661 caaggaggcc gacaaagaga cctacgtcga gcagcacgag atggctgtgg ccaggtactg
      721 cgacctgcct agcaaactgg ggcacagcgg gcatggcacc ggcagcaccg gcagcggcag
      781 ctccggtacc gcctcctccg aggacaacaa catggccatg gtgggtgagg atagcgtgct
      841 gatcaccgag aacatgcaca tgaaactgta catggagggc accgtgaacg accaccactt
      901 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgaagatcaa
      961 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     1021 cggcagcaaa acctttatca accacaccca gggcatcccc gacttcttta agcagtcctt
     1081 ccctgagggc ttcacatggg agaggatcac cacatacgaa gacgggggcg tgctgaccgc
     1141 tacccaggac accagcctcc agaacggctg cctcatctac aacgtcaaga tcaacggggt
     1201 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccagcac
     1261 cgagatgctg taccccgctg acagcggcct gagaggccat gcccagatgg ccctgaagct
     1321 cgtgggcggg ggctacctgc actgctccct caagaccaca tacagatcca agaaacccgc
     1381 taagaacctc aagatgcccg gcttctactt cgtggacagg agactggaaa gaatcaagga
     1441 ggccgacaaa gagacctacg tcgagcagca cgagatggct gtggccaggt actgcgacct
     1501 gcctagcaaa ctggggcaca gctccggact cagatcttga gcggccgcaa gcttaattag
     1561 ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
     1621 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc tagccacctg
     1681 acgtcggggg gggggggaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca
     1741 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     1801 gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa
     1861 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1921 gacaatctat cgcttgtatg ggaagcccca tgcgccagag ttgtttctga aacatggcaa
     1981 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2041 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgactaga gcatgagatt
     2101 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2161 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2221 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgacg tggccgacca
     2281 gcccgtcatc gtcaacgcct gatccgcggt gcggacaggc cgtgtcgcga ccggccgtgc
     2341 ggaattaagc cggcccgtac cctgtgaata gaggtccgct gtgacacaag aatccctgtt
     2401 acttctcgac cgtattgatt cggatgattc ctacgcgagc ctgcggaacg accaggaatt
     2461 ctgggagccg ctggcccgcc gagccctgga ggagctcggg ctgccggtgc cgccggtgct
     2521 gcgggtgccc ggcgagagca ccaaccccgt actggtcggc gagcccggcc cggtgatcaa
     2581 gctgttcggc gagcactggt gcggtccgga gagcctcgcg tcggagtcgg aggcgtacgc
     2641 ggtcctggcg gacgccccgg tgccggtgcc ccgcctcctc ggccgcggcg agctgcggcc
     2701 cggcaccgga gcctggccgt ggccctacct ggtgatgagc cggatgaccg gcaccacctg
     2761 gcggtccgcg atggacggca cgaccgaccg gaacgcgctg ctcgccctgg cccgcgaact
     2821 cggccgggtg ctcggccggc tgcacagggt gccgctgacc gggaacaccg tgctcacccc
     2881 ccattccgag gtcttcccgg aactgctgcg ggaacgccgc gcggcgaccg tcgaggacca
     2941 ccgcgggtgg ggctacctct cgccccggct gctggaccgc ctggaggact ggctgccgga
     3001 cgtggacacg ctgctggccg gccgcgaacc ccggttcgtc cacggcgacc tgcacgggac
     3061 caacatcttc gtggacctgg ccgcgaccga ggtcaccggg atcgtcgact tcaccgacgt
     3121 ctatgcggga gactcccgct acagcctggt gcaactgcat ctcaacgcct tccggggcga
     3181 ccgcgagatc ctggccgcgc tgctcgacgg ggcgcagtgg aagcggaccg aggacttcgc
     3241 ccgcgaactg ctcgccttca ccttcctgca cgacttcgag gtgttcgagg agaccccgct
     3301 ggatctctcc ggcttcaccg atccggagga actggcgcag ttcctctggg ggccgccgga
     3361 caccgccccc ggcgcctgac gccccctcta gtaatctttt gccattctca ccggattcag
     3421 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     3481 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     3541 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     3601 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     3661 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     3721 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     3781 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     3841 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     3901 ctggtatgag tcagcaacac cttcttcacg aggcagacct cactagttcc actgagcgtc
     3961 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4021 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4081 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     4141 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4201 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4261 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4321 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4381 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4441 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4501 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4561 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4621 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4681 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg
     4741 agcccaccag ctccgtaagt tcgggtgctg tgtggctcgt acccgcgcat tcaggcggca
     4801 gggggtctaa cgggtctaag gcggcgtgta cggccgccac agcggctctt agcggcccgg
     4861 aaacgtcctc gaaacgacgc atgtgttcct cctggttggt acaggtggtt gggggtgctc
     4921 ggctgtcgct ggtgtttcat catcagggct cgacgggaga gcgggggagt gtgcagttgt
     4981 ggggtggccc ctcagcgaaa tatctgactt ggagctcgtg tcggaccata caccggtgat
     5041 taatcgtggt ttattatcaa gcgtgagcca cgtcgccgac gaatttgagc agctctggct
     5101 gccgtactgg tccctggcaa gcgacgatct gctcgagggg atctaccgcc aaagccgcgc
     5161 gtcggcccta ggccgccggt acatcgaggc gaacccaaca gcgctggcaa acctgctggt
     5221 cgtggacgta gaccatccag acgcagcgct ccgagcgctc agcgcccggg ggtcccatcc
     5281 gctgcccaac gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag tgtgggcact
     5341 caacgcccct gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat acatggcggc
     5401 gtgcgccgaa ggccttcggc gcgccgtcga tggcgaccgc agttactcag gcctcatgac
     5461 caaaaacccc ggccacatcg cctgggaaac ggaatggctc cactcagatc tctacacact
     5521 cagccacatc gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc gtcagcagac
     5581 cacgtacaaa gcggctccga cgccgctagg gcggaattgc gcactgttcg attccgtcag
     5641 gttgtgggcc tatcttcccg ccctcatgcg gatctacctg ccgacccgga acgtggacgg
     5701 actcggccgc gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc cgtgcaacga
     5761 cgtgtgtccc ggaccgctac cggacagcga ggtccgcgcc atcgccaaca gcatttggcg
     5821 ttggatcaca accaagtcgc gcatttgggc ggacgggatc gtggtctacg aggccacact
     5881 cagtgcgcgc catgcggcca tctcgcggaa gggcgcagca gcgcgcacgg cggcgagcac
     5941 agttgcgcgg cgcgcaaagt ccgcgtcagc catggaggca ttgctatgag cgacggctac
     6001 agcgacggct acagcgacgg ctacaactgg cagccgactg tccgcaaaaa gcggcgcgtg
     6061 accgccgccg aaggcgctcg aatcaccgga ctatccgaac gccacgtcgt ccggctcgtg
     6121 gcgcaggaac gcagcgagtg gttcgccgag caggctgcac gccgcgaacg catccgcgcc
     6181 tatcacgacg acgagggcca ctcttggccg caaacggcca aacatttcgg gctgcatctg
     6241 gacaccgtta agcgactcgg ctatcgggcg aggaaagagc gtgcggcaga acaggaagcg
     6301 gctcaaaagg cccacaacga agccgacaat ccaccgctgt tctaacgcaa ttggggagcg
     6361 ggtgtcgcgg gggttccgtg gggggttccg ttgcaacggg tcggacaggt aaaagtcctg
     6421 gtagacgcta gttttctggt ttgggccatg cctgtctcgt tgcgtgtttc gttgcgtccg
     6481 ttttgaatac cagccagacg agacggggtt ctacgaatct tggtcgatac caagccattt
     6541 ccgctgaata tcgtggagct caccgccaga atcggtggtt gtggtgatgt acgtggcgaa
     6601 ctccgttgta gtgcttgtgg tggcatccgt ggcgcggccg ctctagaact agtggatctg
     6661 acccgctcca caaccgctga gcagggtggc agtcagagcg accaggccga ccgatacgag
     6721 gcggattttg aacaggttca ctcgaagcct cccgctgggt tcggagcatc gtcgatcaca
     6781 tgacggcgtg ctgacaccga ttcgactatg cacagtagta ggtagccgcc gcggacggta
     6841 gcgcaggcgg cctcggcgcc gtcgtggcgt cgcgatggtt ggcgccagac ccgcctgatc
     6901 ggcgtcccga ccagggccgc cggggtggtg agcctcggct tggtccagcc gtagtgagac
     6961 gggatgtctt cccggttgtc gggtttgggt gggcggtcac atgttcgctg gctgtgggcg
     7021 tgtcgggggt cgtcgaagcg gccgcgaaca gggctggacg atgcacgtct tgcacctcct
     7081 gtcaaccccc aatgttgagc cgcgatgccc ctgacctgca ccgttggacc gcagtcgtta
     7141 ggggggcgtg ttagcatgga gtgacgaaag gggcctaaat cagatgatcc cccgggctgc
     7201 aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
//
