LOCUS       sequence-138690-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For analysis of PH domain localization in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray 
            D, Emr SD, Lemmon MA
  TITLE     Genome-wide analysis of membrane targeting by S. cerevisiae 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2004 Mar 12;13(5):677-88.
  PUBMED    15023338
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-03-12"; volume: "13(5)"; pages: "677-88"
COMMENT     Sequence Label: pRS426-GFP-Num1-PH
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..20
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5..21
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(488..508)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccctcgaggt cgacggtatc gataagcttg atatcgaatt cactctaaac ttatcccttg
       61 catgttcctt tgaagtaaat aacgtaatga attataccaa atattgtgtc tttgccttgt
      121 aggacaagta aacttaatag tcctagtttc tgtggtaaca acaatacttt tgtgatacaa
      181 tcctgttgga tatgggtttg ggtctgtgac actttctact cctagaatgg caacaccttt
      241 tgttttggta ttggcaggat tctctaggat gggattagaa gcggaccagt acaaagttaa
      301 ggtatatgga tgaacccaga agaatctttc atgacgtgat tcgaatccaa aaggtcccaa
      361 gcgtggataa tacttaaaca aatattcccc aatgacggtt tgggttaacg cgggtattat
      421 gcttggttcg ttggatctgg gtccggactt gtatagttca tccatgccat gtgtaatccc
      481 agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga
      541 aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
      601 tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct
      661 aattttgaag ttaactttga ttccattctt ttgtttgtct gccatgatgt atacattgtg
      721 tgagttatag ttgtattcca atttgtgtcc aagaatgttt ccatcttctt taaa
//
