LOCUS       sequence-138693-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For analysis of PH domain localization in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray 
            D, Emr SD, Lemmon MA
  TITLE     Genome-wide analysis of membrane targeting by S. cerevisiae 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2004 Mar 12;13(5):677-88.
  PUBMED    15023338
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-03-12"; volume: "13(5)"; pages: "677-88"
COMMENT     Sequence Label: pRS426-GFP-PLCdelta-PH
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..20
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5..21
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(476..496)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccctcgaggt cgacggtatc gataagctta cttctgccgc tggtccatgg agccggagtg
       61 gtggatgatc ttgcgcaggc cctgcaccca gtgctgagcg tcagctggtg atggggcaat
      121 gaggtctagg gtgttgcgct ggtccttgaa gacaatggag aagcatcgat cctcggggat
      181 gtctcgggca aacttctcca ggccttctgt gcggtgtccc atccgtacct cctgaatgtc
      241 ctcgatggag aacagctgcg actccgggga cctcatgacc tttcgagatt cctgccagat
      301 ggtcttgcag tcctcctgta gcttgtagaa gcgttcccta cgccacgagc tggacttcac
      361 cttcagaagc tggctgccct tcagaagggc ctgaaggtcc gggtcatcct ggagcccgtg
      421 agatctgggt ccggacttgt atagttcatc catgccatgt gtaatcccag cagctgttac
      481 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
      541 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
      601 ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa ttttgaagtt
      661 aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg agttatagtt
      721 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc
      781 gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct tgtagttccc
      841 gtcatctt
//
