LOCUS       sequence-13870-e        7119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Takaki K, Davis JM, Winglee K, Ramakrishnan L
  TITLE     Evaluation of the pathogenesis and treatment of Mycobacterium 
            marinum infection in zebrafish.
  JOURNAL   Nat Protoc. 2013 Jun;8(6):1114-24. doi: 10.1038/nprot.2013.068. Epub
            2013 May 16.
  PUBMED    23680983
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.068"; journalName: "Nat Protoc"; date: 
            "2013-06"; volume: "8"; issue: "6"; pages: "1114-24"
COMMENT     Sequence Label: pTEC27
FEATURES             Location/Qualifiers
     source          1..7119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(31..47)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(32..48)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             102..1532
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(1724..1743)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1808..1827)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3918..4506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4407..4426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6569..6585
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6569..6585
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg
       61 taccagatct ttaaatctag atttaagaag gagatataca tatggtgagc aagggcgagg
      121 aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
      181 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      241 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca
      301 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt
      361 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga
      421 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg
      481 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      541 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga
      601 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc
      661 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg
      721 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgg
      781 ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc gaggacaaca
      841 acatggccgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg
      901 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      961 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
     1021 agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga
     1081 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc
     1141 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga
     1201 tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
     1261 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
     1321 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca
     1381 agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac atcacctccc
     1441 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt
     1501 tcctgtacgg catggacgag ctgtacaagt aggcggccgc gactctagaa ttccaactga
     1561 gcgccggtcg ctaccattac caacttgtct ggtgtgctag ccacctgacg tcgggggggg
     1621 ggggaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1681 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     1741 catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat
     1801 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     1861 ttgtatggga agccccatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     1921 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     1981 accatcaagc attttatccg tactcctgat gactagagca tgagattatc aaaaaggatc
     2041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2161 ctatttcgtt catccatagt tgcctgactc cccgacgtgg ccgaccagcc cgtcatcgtc
     2221 aacgcctgat ccgcggtgcg gacaggccgt gtcgcgaccg gccgtgcgga attaagccgg
     2281 cccgtaccct gtgaatagag gtccgctgtg acacaagaat ccctgttact tctcgaccgt
     2341 attgattcgg atgattccta cgcgagcctg cggaacgacc aggaattctg ggagccgctg
     2401 gcccgccgag ccctggagga gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc
     2461 gagagcacca accccgtact ggtcggcgag cccggcccgg tgatcaagct gttcggcgag
     2521 cactggtgcg gtccggagag cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac
     2581 gccccggtgc cggtgccccg cctcctcggc cgcggcgagc tgcggcccgg caccggagcc
     2641 tggccgtggc cctacctggt gatgagccgg atgaccggca ccacctggcg gtccgcgatg
     2701 gacggcacga ccgaccggaa cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc
     2761 ggccggctgc acagggtgcc gctgaccggg aacaccgtgc tcacccccca ttccgaggtc
     2821 ttcccggaac tgctgcggga acgccgcgcg gcgaccgtcg aggaccaccg cgggtggggc
     2881 tacctctcgc cccggctgct ggaccgcctg gaggactggc tgccggacgt ggacacgctg
     2941 ctggccggcc gcgaaccccg gttcgtccac ggcgacctgc acgggaccaa catcttcgtg
     3001 gacctggccg cgaccgaggt caccgggatc gtcgacttca ccgacgtcta tgcgggagac
     3061 tcccgctaca gcctggtgca actgcatctc aacgccttcc ggggcgaccg cgagatcctg
     3121 gccgcgctgc tcgacggggc gcagtggaag cggaccgagg acttcgcccg cgaactgctc
     3181 gccttcacct tcctgcacga cttcgaggtg ttcgaggaga ccccgctgga tctctccggc
     3241 ttcaccgatc cggaggaact ggcgcagttc ctctgggggc cgccggacac cgcccccggc
     3301 gcctgacgcc ccctctagta atcttttgcc attctcaccg gattcagtcg tcactcatgg
     3361 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3421 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3481 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3541 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3601 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata
     3661 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt
     3721 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc
     3781 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca
     3841 gcaacacctt cttcacgagg cagacctcac tagttccact gagcgtcaga ccccgtagaa
     3901 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3961 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4021 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4081 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4141 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4201 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4261 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     4321 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4381 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4441 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4501 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4561 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4621 tgagctgata ccgctcgccg cagccgaacg accgagcgca acgcgtgagc ccaccagctc
     4681 cgtaagttcg ggtgctgtgt ggctcgtacc cgcgcattca ggcggcaggg ggtctaacgg
     4741 gtctaaggcg gcgtgtacgg ccgccacagc ggctcttagc ggcccggaaa cgtcctcgaa
     4801 acgacgcatg tgttcctcct ggttggtaca ggtggttggg ggtgctcggc tgtcgctggt
     4861 gtttcatcat cagggctcga cgggagagcg ggggagtgtg cagttgtggg gtggcccctc
     4921 agcgaaatat ctgacttgga gctcgtgtcg gaccatacac cggtgattaa tcgtggttta
     4981 ttatcaagcg tgagccacgt cgccgacgaa tttgagcagc tctggctgcc gtactggtcc
     5041 ctggcaagcg acgatctgct cgaggggatc taccgccaaa gccgcgcgtc ggccctaggc
     5101 cgccggtaca tcgaggcgaa cccaacagcg ctggcaaacc tgctggtcgt ggacgtagac
     5161 catccagacg cagcgctccg agcgctcagc gcccgggggt cccatccgct gcccaacgcg
     5221 atcgtgggca atcgcgccaa cggccacgca cacgcagtgt gggcactcaa cgcccctgtt
     5281 ccacgcaccg aatacgcgcg gcgtaagccg ctcgcataca tggcggcgtg cgccgaaggc
     5341 cttcggcgcg ccgtcgatgg cgaccgcagt tactcaggcc tcatgaccaa aaaccccggc
     5401 cacatcgcct gggaaacgga atggctccac tcagatctct acacactcag ccacatcgag
     5461 gccgagctcg gcgcgaacat gccaccgccg cgctggcgtc agcagaccac gtacaaagcg
     5521 gctccgacgc cgctagggcg gaattgcgca ctgttcgatt ccgtcaggtt gtgggcctat
     5581 cttcccgccc tcatgcggat ctacctgccg acccggaacg tggacggact cggccgcgcg
     5641 atctatgccg agtgccacgc gcgaaacgcc gaatttccgt gcaacgacgt gtgtcccgga
     5701 ccgctaccgg acagcgaggt ccgcgccatc gccaacagca tttggcgttg gatcacaacc
     5761 aagtcgcgca tttgggcgga cgggatcgtg gtctacgagg ccacactcag tgcgcgccat
     5821 gcggccatct cgcggaaggg cgcagcagcg cgcacggcgg cgagcacagt tgcgcggcgc
     5881 gcaaagtccg cgtcagccat ggaggcattg ctatgagcga cggctacagc gacggctaca
     5941 gcgacggcta caactggcag ccgactgtcc gcaaaaagcg gcgcgtgacc gccgccgaag
     6001 gcgctcgaat caccggacta tccgaacgcc acgtcgtccg gctcgtggcg caggaacgca
     6061 gcgagtggtt cgccgagcag gctgcacgcc gcgaacgcat ccgcgcctat cacgacgacg
     6121 agggccactc ttggccgcaa acggccaaac atttcgggct gcatctggac accgttaagc
     6181 gactcggcta tcgggcgagg aaagagcgtg cggcagaaca ggaagcggct caaaaggccc
     6241 acaacgaagc cgacaatcca ccgctgttct aacgcaattg gggagcgggt gtcgcggggg
     6301 ttccgtgggg ggttccgttg caacgggtcg gacaggtaaa agtcctggta gacgctagtt
     6361 ttctggtttg ggccatgcct gtctcgttgc gtgtttcgtt gcgtccgttt tgaataccag
     6421 ccagacgaga cggggttcta cgaatcttgg tcgataccaa gccatttccg ctgaatatcg
     6481 tggagctcac cgccagaatc ggtggttgtg gtgatgtacg tggcgaactc cgttgtagtg
     6541 cttgtggtgg catccgtggc gcggccgctc tagaactagt ggatctgacc cgctccacaa
     6601 ccgctgagca gggtggcagt cagagcgacc aggccgaccg atacgaggcg gattttgaac
     6661 aggttcactc gaagcctccc gctgggttcg gagcatcgtc gatcacatga cggcgtgctg
     6721 acaccgattc gactatgcac agtagtaggt agccgccgcg gacggtagcg caggcggcct
     6781 cggcgccgtc gtggcgtcgc gatggttggc gccagacccg cctgatcggc gtcccgacca
     6841 gggccgccgg ggtggtgagc ctcggcttgg tccagccgta gtgagacggg atgtcttccc
     6901 ggttgtcggg tttgggtggg cggtcacatg ttcgctggct gtgggcgtgt cgggggtcgt
     6961 cgaagcggcc gcgaacaggg ctggacgatg cacgtcttgc acctcctgtc aacccccaat
     7021 gttgagccgc gatgcccctg acctgcaccg ttggaccgca gtcgttaggg gggcgtgtta
     7081 gcatggagtg acgaaagggg cctaaatcag atgatcccc
//
