LOCUS       sequence-138704-        7718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sema4C protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema4c-Fc-His
FEATURES             Location/Qualifiers
     source          1..7718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4298..4315
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4341..4462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4378..4397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4432..4451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4500..4517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4671..5259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4751..4770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5430..6221,6222..6290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6053..6072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6291..6395)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6463..6481
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6519..6541)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6543..6563)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6548..6878
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6729..6864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6791..6810
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7015..7034
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7101..7556)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7238..7259)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7450..7469
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7682..7704
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7696..7713
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7697..7713
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggccccaca
     1621 ctgggctgtc tggctgctgg cagcagggct gtggggcctg ggcatcgggg ctgagatgtg
     1681 gtggaacctt gtgccccgga agacagtatc ttctggggag ctggtcacag tagtgaggcg
     1741 gttctcccag acaggcatcc aggacttcct gacactgacc ctgacagaac attctggcct
     1801 tttatatgtg ggggcccgag aggcgctgtt tgccttcagt gtagaggctc tggagctgca
     1861 aggagcgatc tcttgggagg ctccagctga gaagaaaatt gaatgtaccc agaaagggaa
     1921 gagcaaccag accgaatgct tcaacttcat ccgcttcctt cagccataca attcctccca
     1981 tctgtatgtc tgcggcacct atgccttcca gcccaagtgc acctacatca acatgctcac
     2041 gttcaccttg gaccgtgcag aatttgagga tgggaagggt aaatgcccat atgacccagc
     2101 taagggtcac accggactcc ttgtggacgg tgagctgtac tcagccacac tcaataactt
     2161 cctgggcaca gagccggtta tccttcgata catggggacc caccactcca tcaagacaga
     2221 gtacctggct ttttggctga atgaacccca ctttgtaggc tctgcctttg tccctgagag
     2281 tgtgggaagc ttcacgggag acgatgacaa gatctacttc ttcttcagtg agcgggcagt
     2341 ggagtatgac tgctattccg agcaggtggt ggctcgtgtg gcgagagtct gtaagggtga
     2401 catgggggga gcacggacgc tgcagaagaa atggacgacg ttcctgaagg ctcggttggt
     2461 gtgctcagcc cctgactgga aggtctactt caaccagctg aaggcggtgc acaccctgcg
     2521 gggcgcctct tggcacaaca ccaccttctt cggggttttt caagcgcgat ggggcgatat
     2581 ggacctgtct gcagtttgtg agtaccagtt ggaacagatc cagcaagtgt ttgagggtcc
     2641 ctacaaggag tacagtgagc aagcccagaa gtgggcccgc tatactgacc cggtacccag
     2701 ccctcggcct ggttcgtgta tcaacaactg gcaccgagac aatggctaca ccagttccct
     2761 ggaactgccg gacaacaccc tcaacttcat caagaagcac cccctgatgg aggaccaggt
     2821 gaagcctcgg ttgggccgcc ccctacttgt gaagaagaac actaacttca cacacgtggt
     2881 ggccgacagg gtcccagggc ttgatggtgc cacctataca gtgttgttca ttggtacagg
     2941 agatggctgg ctgctgaagg ctgtgagcct ggggccctgg atccacatgg tggaggaact
     3001 gcaggtgttt gaccaggagc cagtggaaag tctggtgctg tctcagagca agaaggtgct
     3061 ctttgctggc tcccgctctc agctggttca gctgtctctg gccgactgca caaagtaccg
     3121 tttctgtgta gactgtgtcc tggccaggga cccttactgt gcctggaatg tcaacaccag
     3181 ccgctgtgtg gccaccacca gtggtcgctc ggggtccttt ctggtccaac atgtggcgaa
     3241 cttggacact tcaaagatgt gtaaccagta tggcattaaa aaagtcagat ctattcccaa
     3301 gaacatcacc gttgtgtcag gcacagacct ggtcctaccc tgccacctct cgtccaattt
     3361 ggcccatgcc cactggacct tcggaagcca ggacctgcct gcagaacaac ctggctcctt
     3421 tctttatgac acgggactcc aggcgctggt ggtgatggcc gcacagtccc gtcactctgg
     3481 accctatcgt tgctattcag aggagcaggg gacaagactg gctgcagaaa gctaccttgt
     3541 tgctgtcgtg gccggctcgt cggtgacact ggaggcacgg gctcccttgg aaaacctggg
     3601 gggcgcgcct gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg
     3661 accgtcagtc ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc
     3721 tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg
     3781 gtacgtggac ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa
     3841 cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac caggactggc tgaatggcaa
     3901 ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc
     3961 caaagccaaa gggcagcccc gagaaccaca ggtgtatacc ctgcccccat cccgggatga
     4021 gctgaccaag aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat
     4081 cgccgtggag tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt
     4141 gctggactcc gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg
     4201 gcagcagggg aacgtcttct catgctccgt gatgcatgag gctctgcaca accactacac
     4261 gcagaagagc ctctccctgt ctccgggtaa aggcggccat catcaccatc accattaatc
     4321 tagagatctt attaaagcag aacttgttta ttgcagctta taatggttac aaataaagca
     4381 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4441 ccaaactcat caatgtatct tatcatgtct ggtcgactct agctagactc ttccgcttcc
     4501 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4561 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4621 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4681 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4741 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4801 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4861 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4921 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4981 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5041 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5101 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5161 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5341 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5401 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5461 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5521 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5581 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5641 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5701 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5761 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5821 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5881 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5941 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6001 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6061 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6361 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6421 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6481 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6541 gaggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     6601 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6661 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6721 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6781 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6841 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     6901 ccattttcgt cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     6961 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     7021 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     7081 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     7141 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     7201 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     7261 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     7321 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     7381 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     7441 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     7501 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     7561 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7621 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     7681 tcccagtcac gacgttgtaa aacgacggcc agtgaatt
//
