LOCUS       sequence-138719-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFUGW-EFSp-Cas9-P2A-Zeo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp30
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..49
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gacgcgggat ctagaggcca ccatggcccc aaagaagaag cggaaggtcg gtatccacgg
       61 agtcccagca gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg
      121 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
      181 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
      241 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
      301 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
      361 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
      421 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
      481 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
      541 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
      601 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
      661 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
      721 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
      781 gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
      841 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acct
//
