LOCUS       sequence-138725-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp28
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(49..124)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(159..407)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(217..236)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(387..407)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ttaacaacaa caattgtgcg tcaggaattc cgttgcggat ccaaaaaagc accgactcgg
       61 tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt tctagctcta
      121 aaacaggtct tcgctgcact cgagtgcagc gaagacccgg tgtttcgtcc tttccacaag
      181 atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt
      241 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
      301 ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc tctaacagcc
      361 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctctag atcttcagcc
      421 taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
      481 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
      541 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
      601 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccccgt
      661 gcactcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
      721 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
      781 cacgctgccg gctcg
//
