LOCUS       sequence-138728-        1602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of N-terminal His-tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Mullarky E, Lucki NC, Beheshti Zavareh R, Anglin JL, Gomes AP, 
            Nicolay BN, Wong JC, Christen S, Takahashi H, Singh PK, Blenis J, 
            Warren JD, Fendt SM, Asara JM, DeNicola GM, Lyssiotis CA, Lairson 
            LL, Cantley LC
  TITLE     Identification of a small molecule inhibitor of 3-phosphoglycerate 
            dehydrogenase to target serine biosynthesis in cancers.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):1778-83. doi: 
            10.1073/pnas.1521548113. Epub 2016 Feb 1.
  PUBMED    26831078
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521548113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-02-16- 16"; volume: "113"; issue: "7"; pages: "1778-83"
COMMENT     Sequence Label: pET28a-PHGDH
FEATURES             Location/Qualifiers
     source          1..1602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcttttg caaatctgcg gaaagtgctc atcagtgaca gcctggaccc ttgctgccgg
       61 aagatcttgc aagatggagg gctgcaggtg gtggaaaagc agaaccttag caaagaggag
      121 ctgatagcgg agctgcagga ctgtgaaggc cttattgttc gctctgccac caaggtgacc
      181 gctgatgtca tcaacgcagc tgagaaactc caggtggtgg gcagggctgg cacaggtgtg
      241 gacaatgtgg atctggaggc cgcaacaagg aagggcatct tggttatgaa cacccccaat
      301 gggaacagcc tcagtgccgc agaactcact tgtggaatga tcatgtgcct ggccaggcag
      361 attccccagg cgacggcttc gatgaaggac ggcaaatggg agcggaagaa gttcatggga
      421 acagagctga atggaaagac cctgggaatt cttggcctgg gcaggattgg gagagaggta
      481 gctacccgga tgcagtcctt tgggatgaag actatagggt atgaccccat catttcccca
      541 gaggtctcgg cctcctttgg tgttcagcag ctgcccctgg aggagatctg gcctctctgt
      601 gatttcatca ctgtgcacac tcctctcctg ccctccacga caggcttgct gaatgacaac
      661 acctttgccc agtgcaagaa gggggtgcgt gtggtgaact gtgcccgtgg agggatcgtg
      721 gacgaaggcg ccctgctccg ggccctgcag tctggccagt gtgccggggc tgcactggac
      781 gtgtttacgg aagagccgcc acgggaccgg gccttggtgg accatgagaa tgtcatcagc
      841 tgtccccacc tgggtgccag caccaaggag gctcagagcc gctgtgggga ggaaattgct
      901 gttcagttcg tggacatggt gaaggggaaa tctctcacgg gggttgtgaa tgcccaggcc
      961 cttaccagtg ccttctctcc acacaccaag ccttggattg gtctggcaga agctctgggg
     1021 acactgatgc gagcctgggc tgggtccccc aaagggacca tccaggtgat aacacaggga
     1081 acatccctga agaatgctgg gaactgccta agccccgcag tcattgtcgg cctcctgaaa
     1141 gaggcttcca agcaggcgga tgtgaacttg gtgaacgcta agctgctggt gaaagaggct
     1201 ggcctcaatg tcaccacctc ccacagccct gctgcaccag gggagcaagg cttcggggaa
     1261 tgcctcctgg ccgtggccct ggcaggcgcc ccttaccagg ctgtgggctt ggtccaaggc
     1321 actacgcctg tactgcaggg gctcaatgga gctgtcttca ggccagaagt gcctctccgc
     1381 agggacctgc ccctgctcct attccggact cagacctctg accctgcaat gctgcctacc
     1441 atgattggcc tcctggcaga ggcaggcgtg cggctgctgt cctaccagac ttcactggtg
     1501 tcagatgggg agacctggca cgtcatgggc atctcctcct tgctgcccag cctggaagcg
     1561 tggaagcagc atgtgactga agccttccag ttccacttct aa
//
