LOCUS       sequence-138731-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp9
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             119..814
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa
       61 ttgtccgcta aattctggcc gtttttggct tttttgttag acagttaatt aagccaccat
      121 gagcgagctg atcaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa
      181 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
      241 gaagatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag
      301 cttcatgtac ggcagcaaag ccttcatcaa ccacacccag ggcatccccg acttctttaa
      361 gcagtccttc cctgagggct tcacatggga gagaatcacc acatacgaag acgggggcgt
      421 gctgaccgct acccaggaca ccagcttcca gaacggctgc atcatctaca acgtcaagat
      481 caacggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac gcggctggga
      541 ggccaacacc gagatgctgt accccgctga cggcggcctg agaggccaca gccagatggc
      601 cctgaagctc gtgggcgggg gctacctgca ctgctccttc aagaccacat acagatccaa
      661 gaaacccgct aagaacctca agatgcccgg cttccacttc gtggaccaca gactggaaag
      721 aatcaaggag gccgacaaag agacctacgt cgagcagcac gagatggctg tggccaagta
      781 ctgcgacctc cctagcaaac tggggcacag ataataacct gcaggcgggc cagatatacg
      841 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacg
//
