LOCUS       sequence-138734-       11252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp9
FEATURES             Location/Qualifiers
     source          1..11252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        28..407
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..611
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      624..633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(630..632,633..635,636..1349)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(630..632,633..635,636..1346)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(675..696)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(936..955)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1283..1304
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1408..1996
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1461..1481)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1879..1890)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1999..2015)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2000..2016)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2521..2701
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2727..2744)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2733..2957
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3003..3431
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3090..3109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3300..3321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3440..3460)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3445..3774
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3625..3760
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3687..3706
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3822..3869
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3888..4262
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4392..4513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4429..4448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4483..4502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4562..4578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4562..4578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4575..4597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4586..4602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4610..4616,4617..4634,4635..4640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4655..4676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4793..4810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4964..5552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5044..5063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5723..6514,6515..6583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6346..6365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6584..6688)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6763..6782)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6954..7333
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7335..7533
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7551..7731
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7778..7903
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8396..8629
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8814..8858
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9156..9273
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9327..10537
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     10374..10393
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             10554..11249
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 cctgcaggcg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagcgg taacctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
      661 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      721 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      781 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      841 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      901 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      961 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1021 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1081 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1141 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1201 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1261 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1321 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctaggatc cgcgaaaagt
     1381 ccggaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     1441 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1501 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     1561 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     1621 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     1681 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     1741 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     1801 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     1861 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     1921 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     1981 tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc
     2041 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     2101 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     2161 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     2221 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     2281 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     2341 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     2401 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     2461 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     2521 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2581 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2641 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     2701 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     2761 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     2821 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     2881 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     2941 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     3001 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3061 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3361 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3421 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     3481 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     3541 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3601 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3661 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     3721 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     3781 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     3841 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     3901 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     3961 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     4021 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     4081 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     4141 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     4201 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     4261 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4321 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4381 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4501 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     4561 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4621 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4681 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4741 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5401 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5581 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5641 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5701 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5761 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5821 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5881 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5941 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6001 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6061 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6121 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6181 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6241 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6301 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6361 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6421 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6481 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6541 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6601 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6661 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     6721 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     6781 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     6841 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     6901 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     6961 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     7021 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     7081 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     7141 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     7201 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     7261 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     7321 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     7381 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     7441 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     7501 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     7561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     7741 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     7801 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     7861 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     7921 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     7981 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     8041 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     8101 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     8161 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     8221 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     8281 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     8341 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     8401 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     8461 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     8521 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8581 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     8641 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     8701 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     8761 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     8821 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     8881 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     8941 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9001 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     9061 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     9121 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     9181 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     9241 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     9301 ccagtttggt taattaaggg tgcagcggcc tccgcgccgg gttttggcgc ctcccgcggg
     9361 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagga gcgttcctga
     9421 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     9481 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     9541 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     9601 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     9661 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     9721 cttggtgagt tgcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
     9781 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
     9841 ccctgaactg ggggttgggg ggagcgcaca aaatggcggc tgttcccgag tcttgaatgg
     9901 aagacgcttg taaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     9961 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
    10021 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcgggt
    10081 ttgtcgtctg gttgcggggg cggcagttat gcggtgccgt tgggcagtgc acccgtacct
    10141 ttgggagcgc gcgcctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
    10201 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
    10261 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
    10321 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
    10381 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
    10441 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
    10501 cgctaaattc tggccgtttt tggctttttt gttagacagt taattaagcc accatgagcg
    10561 agctgatcaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc
    10621 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgaaga
    10681 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
    10741 tgtacggcag caaagccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt
    10801 ccttccctga gggcttcaca tgggagagaa tcaccacata cgaagacggg ggcgtgctga
    10861 ccgctaccca ggacaccagc ttccagaacg gctgcatcat ctacaacgtc aagatcaacg
    10921 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacacgcggc tgggaggcca
    10981 acaccgagat gctgtacccc gctgacggcg gcctgagagg ccacagccag atggccctga
    11041 agctcgtggg cgggggctac ctgcactgct ccttcaagac cacatacaga tccaagaaac
    11101 ccgctaagaa cctcaagatg cccggcttcc acttcgtgga ccacagactg gaaagaatca
    11161 aggaggccga caaagagacc tacgtcgagc agcacgagat ggctgtggcc aagtactgcg
    11221 acctccctag caaactgggg cacagataat aa
//
