LOCUS       sequence-138735-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP-U6p-GFP-sg1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp9-1
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             123..818
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag
       61 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacagtt aattaagcca
      121 ccatgagcga gctgatcaag gagaacatgc acatgaagct gtacatggag ggcaccgtga
      181 acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
      241 ccatgaagat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
      301 ccagcttcat gtacggcagc aaagccttca tcaaccacac ccagggcatc cccgacttct
      361 ttaagcagtc cttccctgag ggcttcacat gggagagaat caccacatac gaagacgggg
      421 gcgtgctgac cgctacccag gacaccagct tccagaacgg ctgcatcatc tacaacgtca
      481 agatcaacgg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acacgcggct
      541 gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctgagaggc cacagccaga
      601 tggccctgaa gctcgtgggc gggggctacc tgcactgctc cttcaagacc acatacagat
      661 ccaagaaacc cgctaagaac ctcaagatgc ccggcttcca cttcgtggac cacagactgg
      721 aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgagatg gctgtggcca
      781 agtactgcga cctccctagc aaactggggc acagataata acctgcaggc gggccagata
      841 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      901 tcatagccca tatat
//
