LOCUS       sequence-138736-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP-U6p-GFP-sg1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp9-1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(74..149)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(171..419)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(229..248)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(399..419)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(488..509)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 taatccagag gttgattatc gataagcttg atatcgaatt gtgcgtcagg aattccgttg
       61 cggatccaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta
      121 ttttaacttg ctatttctag ctctaaaacc ggtgaacagc tcctcgcccc ggtgtttcgt
      181 cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata
      241 tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt
      301 ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat tctaattatc
      361 tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctct
      421 agatcctaga gtcgcggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg
      481 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      541 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      601 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      661 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      721 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      781 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgg
//
